猪胸膜肺炎放线杆菌的药敏性及质粒分离

Drug-susceptibility and isolation of a plasmid in Haemophilus (Actinobacillus) pleuropneumoniae.

作者信息

Ishii H, Nakasone Y, Shigehara S, Honma K, Araki Y, Iyobe S, Hashimoto H

机构信息

Central Meat Inspection Laboratory, Prefecture of Gunma, Japan.

出版信息

Nihon Juigaku Zasshi. 1990 Feb;52(1):1-9. doi: 10.1292/jvms1939.52.1.

DOI:10.1292/jvms1939.52.1

PMID:1968988

Abstract

We isolated 56 Haemophilus (Actinobacillus) pleuropneumoniae strains from the pneumonic porcine lung tissues and tested them for antimicrobial susceptibility. Two drug-resistant strains were obtained. One, named KH-265, was resistant to streptomycin (SM) and sulfonamide (SA), and the other, named KH-195, was resistant to tetracycline (TC). The minimum inhibitory concentrations (MICs) of drugs for resistant strains were 100 micrograms/mliters for SM, 3200 micrograms/mliters for SA, and 12.5 micrograms/mliters for TC. KH-265 possessed a 8.3Kb nonconjugative plasmid, pMS260, encoding SM and SA resistance, which was transformable to E. coli strains. pMS260 belonged to none of 14 incompatibility groups including Inc. P and Inc. Q, so far tested. It was mobilizable to various causative strains for respiratory infections, Pseudomonas aeruginosa, Bordetella bronchiseptica, Pasteurella multocida and Haemophilus pleuropneumoniae, by RP4 (Inc. P) plasmid.

摘要

我们从患肺炎的猪肺组织中分离出56株胸膜肺炎放线杆菌，并对其进行药敏试验。获得了两株耐药菌株。一株名为KH - 265，对链霉素（SM）和磺胺类药物（SA）耐药；另一株名为KH - 195，对四环素（TC）耐药。耐药菌株对药物的最低抑菌浓度（MIC）分别为：SM为100微克/毫升，SA为3200微克/毫升，TC为12.5微克/毫升。KH - 265携带一个8.3Kb的非接合质粒pMS260，编码对SM和SA的抗性，该质粒可转化至大肠杆菌菌株。到目前为止，pMS260不属于包括Inc.P和Inc.Q在内的14个不相容群中的任何一个。它可通过RP4（Inc.P）质粒转移至各种呼吸道感染病原菌，如铜绿假单胞菌、支气管败血波氏杆菌、多杀性巴氏杆菌和胸膜肺炎放线杆菌。