Rutledge Claire E, Millar Jocelyn G, Romero Carmen M, Hanks Lawrence M
Department of Entomology, The Connecticut Agricultural Experiment Station, New Haven, CT 06504, USA.
Environ Entomol. 2009 Aug;38(4):1267-75. doi: 10.1603/022.038.0438.
Adult male and female Callidiellum rufipenne (Motschulsky) (Coleoptera: Cerambycidae) aggregate on severely stressed or fallen trees and cut logs of cypress species (Cupressaceae) that are the hosts of their larvae. Our studies showed that male C. rufipenne actively search for females on these trees and only respond to females after contacting them with their antennae. Removing cuticular hydrocarbons from fresh carcasses of females with solvent rendered the carcasses unattractive to males, but activity was restored when the solvent extract was reapplied. These findings suggest that contact pheromones mediate mate recognition in this species. Bioassays of fractions of the extracts determined that the branched chain hydrocarbon fraction was primarily responsible for activity. 5,17-Dimethylnonacosane was a key sex-specific component in extracts of females, and synthetic 5,17-dimethylnonacosane elicited a strong mating response from males. We conclude that this compound is an important component of the contact sex pheromone of C. rufipenne if not the sole component. Solvent extracts of both sexes also contained 9-pentacosyne and 9-heptacosyne. To our knowledge, this is only the second report of alkynes in the cuticular lipids of insects.
成年雄性和雌性红翅长角天牛（Callidiellum rufipenne (Motschulsky)）（鞘翅目：天牛科）聚集在严重受压或倒下的树木以及柏树（柏科）的原木上，这些是它们幼虫的寄主。我们的研究表明，雄性红翅长角天牛会在这些树上积极寻找雌性，并且只有在用触角接触到雌性后才会做出反应。用溶剂去除雌性新鲜尸体上的表皮碳氢化合物会使尸体对雄性失去吸引力，但重新涂抹溶剂提取物后活性得以恢复。这些发现表明接触性信息素介导了该物种的配偶识别。提取物各部分的生物测定确定支链碳氢化合物部分主要负责活性。5,17 - 二甲基二十九烷是雌性提取物中的关键性别特异性成分，合成的5,17 - 二甲基二十九烷引发了雄性强烈的交配反应。我们得出结论，该化合物即使不是唯一成分，也是红翅长角天牛接触性信息素的重要组成部分。两性的溶剂提取物中还含有9 - 二十五炔和9 - 二十七炔。据我们所知，这是昆虫表皮脂质中炔烃的第二份报告。
