Department of Human Genetics, University of Aarhus, Aarhus, Denmark.
Mol Biol Rep. 2010 Jun;37(5):2407-13. doi: 10.1007/s11033-009-9751-8. Epub 2009 Aug 19.
The intermediate filament protein vimentin is involved in a variety of cellular functions both during the normal developmental processes and in human malignancies. We here describe the identification of an alternative vimentin transcript initiating upstream for the canonical vimentin gene promoter and spliced using the vimentin promoter sequence as an intron. Expression analysis showed that the alternative vimentin promoter had the same expression profile as the canonical vimentin gene promoter. The presented data suggest that alternative promoter usage and alternative splicing could be regulatory mechanisms participating in vimentin gene regulation.
中间丝蛋白波形蛋白参与多种细胞功能，无论是在正常发育过程中还是在人类恶性肿瘤中。在这里，我们描述了一种替代的波形蛋白转录本的鉴定，该转录本起始于经典的波形蛋白基因启动子上游，并使用波形蛋白启动子序列作为内含子进行拼接。表达分析表明，替代的波形蛋白启动子与经典的波形蛋白基因启动子具有相同的表达谱。所提供的数据表明，替代启动子的使用和替代剪接可能是参与波形蛋白基因调控的调节机制。
