Teeuwsen V J, Siebelink K H, de Wolf F, Goudsmit J, UytdeHaag F G, Osterhaus A D
Department of Immunobiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
AIDS. 1990 Jan;4(1):77-81. doi: 10.1097/00002030-199001000-00011.
In a previous study we have shown that peripheral blood mononuclear cells (PBMC) from asymptomatic HIV-seropositive male homosexuals, who had seroconverted more than 2 years before, were unable to mount a secondary immune response in vitro to certain viral and bacterial antigens. We have extended this study by investigating the secondary immune responses of five male homosexuals, who, by regular screening, were found to have seroconverted for HIV-1 during the preceding 3 months and were subsequently vaccinated with tetanus toxoid and poliovirus vaccine. Six weeks after the booster vaccination, PBMC of the five recently seroconverted individuals were assayed for in vitro mitogen or recall antigen-induced antibody synthesis and lymphocyte proliferation. The results of this study indicate that certain of the in vitro abnormalities of immune reactions, observed in both symptomatic and asymptomatic HIV-seropositive individuals, can already be found within 3 months after seroconversion.
在之前的一项研究中，我们已经表明，来自无症状HIV血清阳性男性同性恋者的外周血单核细胞（PBMC），这些人在两年多以前血清转换，在体外对某些病毒和细菌抗原无法产生二次免疫应答。我们通过研究五名男性同性恋者的二次免疫应答扩展了这项研究，这些男性通过定期筛查，发现在前3个月内发生了HIV-1血清转换，随后接种了破伤风类毒素和脊髓灰质炎病毒疫苗。加强免疫接种六周后，对这五名近期血清转换者的PBMC进行体外有丝分裂原或回忆抗原诱导的抗体合成及淋巴细胞增殖检测。这项研究结果表明，在有症状和无症状HIV血清阳性个体中观察到的某些体外免疫反应异常，在血清转换后3个月内就已经可以发现。
