Hua Wang, Jiang Jianbin, Rong Xing, Wu Rongzhou, Qiu Huixian, Zhang Yuanhai, Chen Qi
Department of Pediatric Cardiology, Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical College, Wenzhou 325027, PR China.
Biochem Biophys Res Commun. 2009 Oct 23;388(3):595-600. doi: 10.1016/j.bbrc.2009.08.064. Epub 2009 Aug 18.
The present study found that serum H2S level, H2S production rate, CSE mRNA and CSE protein levels were increased in CVB3-induced myocarditis. dl-proparglygylcine (PAG), an irreversible CSE inhibitor, decreased the infected myocardium titers on postinfection day 4, while NaHS, a H2S donor, alleviated myocardial injury and necrosis, inflammatory cell infiltration and interstitial edema on postinfection day 10. These data reveal that the CSE/H2S pathway is upregulated in the heart in a murine model of CVB3-induced myocarditis and that inhibition of endogenous H2S is beneficial to treatment early in the disease while administration of exogenous H2S is protective to infected myocardium during the later stage.
本研究发现，在柯萨奇病毒B3（CVB3）诱导的心肌炎中，血清硫化氢（H2S）水平、H2S产生率、胱硫醚-γ-裂解酶（CSE）信使核糖核酸（mRNA）及CSE蛋白水平均升高。不可逆的CSE抑制剂dl-炔丙基甘氨酸（PAG）可降低感染后第4天受感染心肌的滴度，而H2S供体硫氢化钠（NaHS）则可减轻感染后第10天的心肌损伤与坏死、炎性细胞浸润及间质水肿。这些数据表明，在CVB3诱导的心肌炎小鼠模型中，心脏中的CSE/H2S通路被上调，内源性H2S的抑制在疾病早期对治疗有益，而外源性H2S的给予在后期对受感染心肌具有保护作用。
