Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, 92 APC Road, Kolkata 700009, India.
Biol Trace Elem Res. 2010 Jun;135(1-3):283-94. doi: 10.1007/s12011-009-8497-4. Epub 2009 Aug 21.
Energy dispersive X-ray fluorescence (ED-XRF) technique has been used for the determination of trace element profile during different developmental stages of somatic embryogenic callus of an economically important medicinal plant, Plantago ovata Forssk. Somatic embryogenesis is a plant tissue culture-based technique, which is used for plant regeneration and crop improvement. In the present investigation, elemental content was analysed using ED-XRF technique during different developmental stages and also determine the effect of additives--casein hydrolysate and coconut water on the trace elemental profile of embryogenic callus tissue of P. ovata. Subsequent experiments showed significant alteration in the concentration of K, Ca, Mn, Fe, Zn, Cu, Br, and Sr in both the embryogenic and non-embryogenic callus. Higher K, Ca, Fe, Cu, and Zn accumulation was in embryogenic tissue stage compared to other stages, suggesting these elements are crucial for successful embryogenesis. The results suggest that this information could be useful for formulating a media for in vitro embryo induction of P. ovata.
能量色散 X 射线荧光（ED-XRF）技术已被用于测定体细胞胚性愈伤组织不同发育阶段的痕量元素分布，该愈伤组织来自于一种具有经济重要性的药用植物车前草（Plantago ovata Forssk）。体细胞胚胎发生是一种基于植物组织培养的技术，用于植物再生和作物改良。在本研究中，使用 ED-XRF 技术在不同的发育阶段分析元素含量，并确定添加物——酪蛋白水解物和椰子水对车前草胚性愈伤组织痕量元素分布的影响。随后的实验表明，在胚性和非胚性愈伤组织中，K、Ca、Mn、Fe、Zn、Cu、Br 和 Sr 的浓度都发生了显著变化。与其他阶段相比，胚性组织阶段的 K、Ca、Fe、Cu 和 Zn 积累更高，表明这些元素对成功的胚胎发生至关重要。结果表明，这些信息可能有助于为车前草的体外胚胎诱导制定培养基。
