Ye Yufu, Zhou Lin, Xie Xiaojun, Jiang Guoping, Xie Haiyang, Zheng Shusen
Key Lab of Combined Multi-organ Transplantation, Ministry of Public Health, Department of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
J Surg Oncol. 2009 Nov 1;100(6):500-4. doi: 10.1002/jso.21376.
The B7-H1/PD-1 pathway has recently been found to contribute to immune evasion of cancer cells from host immune system. This study aimed to investigate the expression of B7-H1 and its receptor PD-1 and to explore their significance in the progression of intraheptic cholangiocarcinoma (ICC).
Thirty-one surgically resected ICC tissues and the corresponding cancer adjacent tissues were enrolled from 2006 to 2007. Immunohistochemical studies were performed with antibody of B7-H1, PD-1, CD8, and CD4. Apoptosis status of tumor-infiltrating lymphocytes (TILs) was detected by TUNEL assay.
Expression of B7-H1 and PD-1 was found to be up-regulated in ICC tissues compared with the cancer adjacent tissues. Tumor-related B7-H1 expression was significantly correlated with both tumor differentiation and pTNM stage and was inversely correlated with CD8+ TILs but not CD4+ TILs. TILs in primary carcinoma showed a high level of apoptosis.
B7-H1/PD-1 pathway may be linked to malignant potential of ICC and contribute to tumor immune evasion by promoting CD8+ TILs apoptosis. Thus, this pathway may indeed be a potential therapeutic target in the treatment of this disease.
最近发现B7-H1/PD-1通路有助于癌细胞逃避宿主免疫系统的免疫监视。本研究旨在探讨B7-H1及其受体PD-1的表达情况，并探讨它们在肝内胆管癌（ICC）进展中的意义。
选取2006年至2007年间手术切除的31例ICC组织及其相应的癌旁组织。采用B7-H1、PD-1、CD8和CD4抗体进行免疫组织化学研究。通过TUNEL法检测肿瘤浸润淋巴细胞（TILs）的凋亡状态。
与癌旁组织相比，ICC组织中B7-H1和PD-1的表达上调。肿瘤相关的B7-H1表达与肿瘤分化和pTNM分期显著相关，与CD8+TILs呈负相关，与CD4+TILs无相关性。原发性癌中的TILs显示出高水平的凋亡。
B7-H1/PD-1通路可能与ICC的恶性潜能相关，并通过促进CD8+TILs凋亡导致肿瘤免疫逃逸。因此，该通路确实可能是治疗这种疾病的潜在治疗靶点。
