Fan Wenzhe, Zhang Yu, Carr Peter W, Rutan Sarah C, Dumarey Melanie, Schellinger Adam P, Pritts Wayne
University of Minnesota, Department of Chemistry, 207 Pleasant Street SE, Minneapolis, MN 55455, USA.
J Chromatogr A. 2009 Sep 18;1216(38):6587-99. doi: 10.1016/j.chroma.2009.07.048. Epub 2009 Aug 6.
Fourteen judiciously selected reversed phase columns were tested with 18 cationic drug solutes under the isocratic elution conditions advised in the Snyder-Dolan (S-D) hydrophobic subtraction method of column classification. The standard errors (S.E.) of the least squares regressions of logk' vs. logk'(REF) were obtained for a given column against a reference column and used to compare and classify columns based on their selectivity. The results are consistent with those obtained with a study of the 16 test solutes recommended by Snyder and Dolan. To the extent these drugs are representative, these results show that the S-D classification scheme is also generally applicable to pharmaceuticals under isocratic conditions. That is, those columns judged to be similar based on the 16 S-D solutes were similar based on the 18 drugs; furthermore those columns judged to have significantly different selectivities based on the 16 S-D probes appeared to be quite different for the drugs as well. Given that the S-D method has been used to classify more than 400 different types of reversed phases the extension to cationic drugs is a significant finding.
在Snyder-Dolan（S-D）柱分类疏水减法方法所建议的等度洗脱条件下，对14根经过审慎挑选的反相柱进行了测试，使用了18种阳离子药物溶质。针对给定的柱子与一根参比柱，获得了logk'对logk'(REF)的最小二乘回归的标准误差（S.E.），并用于根据其选择性对柱子进行比较和分类。结果与对Snyder和Dolan推荐的16种测试溶质进行研究所得的结果一致。就这些药物具有代表性而言，这些结果表明，S-D分类方案在等度条件下通常也适用于药物。也就是说，基于16种S-D溶质被判定为相似的那些柱子，基于这18种药物也是相似的；此外，基于16种S-D探针被判定具有显著不同选择性的那些柱子，对于这些药物而言似乎也有很大差异。鉴于S-D方法已被用于对400多种不同类型的反相柱进行分类，将其扩展到阳离子药物是一项重要发现。
