Toxicología, Facultad de Ciencias Exactas, Universidad Nacional de La Plata UNLP, La Plata, Argentina.
Toxicon. 2010 Feb-Mar;55(2-3):333-42. doi: 10.1016/j.toxicon.2009.08.008. Epub 2009 Aug 20.
The effects of MC-LR on antioxidant system in liver and kidney and its effects on hepatic lipid composition after prolonged exposure to sublethal doses of microcystins (MCs) were studied in mice. Mice were treated i.p. with 25 microg of MC-LR/kg body weight or saline solution every 2 days for a month (inflictive stage), then progression or recovery was studied for 1 and 2 months of wash-out. During the inflictive stage, MC-LR-induced oxidative damage and significant changes in liver lipids of treated mice were compared with control mice. A clear dependence of the enzyme defense system was demonstrated with reduced glutathione and alpha-tocopherol availabilities and a concomitant elevation in NOx production. Sub-chronic MC-LR toxicosis produced alterations in lipid components that included a decreased EFA/non-EFA, SFA/PUFA, and n-3/n-6 ratios all of which exhibited a pattern of slow recovery during the recovery periods.
本研究旨在探讨亚致死剂量微囊藻毒素（MCs）长期暴露后 MC-LR 对小鼠肝、肾抗氧化系统的影响及其对肝脂组成的影响。采用腹腔注射方式，以 25μg MC-LR/kg 体重或生理盐水每 2 天 1 次，连续处理 1 个月（攻击阶段），然后进行 1 个月和 2 个月的洗脱期观察。在攻击阶段，比较了 MC-LR 诱导的氧化损伤和实验组小鼠肝脏脂质的显著变化与对照组。研究结果表明，酶防御系统存在明显的依赖性，包括谷胱甘肽和α-生育酚的可用性降低，同时 NOx 生成增加。亚慢性 MC-LR 中毒导致脂质成分发生改变，包括必需脂肪酸/非必需脂肪酸、饱和脂肪酸/多不饱和脂肪酸和 n-3/n-6 比值降低，所有这些比值在恢复期均呈现出缓慢恢复的趋势。
