Knapen Dries, De Wolf Hans, Knaepkens Guy, Bervoets Lieven, Eens Marcel, Blust Ronny, Verheyen Erik
University of Antwerp, Department of Biology, Ecophysiology, Biochemistry and Toxicology Research Group, Groenenborgerlaan 171, 2020 Antwerpen, Belgium.
Aquat Toxicol. 2009 Oct 19;95(1):17-26. doi: 10.1016/j.aquatox.2009.07.022. Epub 2009 Aug 5.
This study presents the results of a microsatellite and allozyme analysis on natural populations of the gudgeon (Gobio gobio) located in a pollution gradient of cadmium and zinc. Differences among contaminated and reference populations were observed at 2 allozyme loci, as well as a relationship between the fish condition factor and glucose-6-phosphate dehydrogenase genotypes, the locus that showed the largest difference in allele frequencies. The microsatellite data partly confirmed the differentiation pattern that was revealed by the allozyme survey. Our data further suggest that at least 2 microsatellite loci may be affected by natural selection. We thus illustrate that both microsatellite and allozyme loci do not necessarily behave as selectively neutral markers in polluted populations. Estimates of population differentiation can therefore be significantly different depending on which loci are being studied. Finally, these results are discussed in the light of the conservation unit concept, because microsatellites are often used to assess genetic variation in endangered natural populations and to propose measures for conservation or management.
本研究展示了对处于镉和锌污染梯度中的 gudgeon（Gobio gobio）自然种群进行微卫星和等位酶分析的结果。在2个等位酶位点观察到了受污染种群与对照种群之间的差异，同时还发现了鱼类状况因子与葡萄糖 - 6 - 磷酸脱氢酶基因型之间的关系，该位点在等位基因频率上显示出最大差异。微卫星数据部分证实了等位酶调查所揭示的分化模式。我们的数据进一步表明，至少有2个微卫星位点可能受到自然选择的影响。因此，我们说明在受污染种群中，微卫星和等位酶位点不一定表现为选择性中性标记。因此，根据所研究的位点不同，种群分化的估计值可能会有显著差异。最后，鉴于保护单元概念对这些结果进行了讨论，因为微卫星常常被用于评估濒危自然种群的遗传变异，并提出保护或管理措施。
