Bohn M C, Kanuicki M
Department of Neurobiology and Anatomy, University of Rochester Medical Center, New York 14642.
J Neurosci Res. 1990 Mar;25(3):281-6. doi: 10.1002/jnr.490250303.
A rodent model of Parkinson's disease, the 1-methyl-4-(2'-methylphenyl)-1,2,3,6-tetrahydropyridine treated mouse, was used to determine whether striatal dopamine levels recover following grafting adrenal medulla into the striatum. Four types of grafts were performed: 1) adult mouse adrenal medulla, 2) adult adrenal medulla that had been freeze-thawed to kill viable cells, 3) postnatal day 7 adrenal medulla, and 4) sham grafts lacking tissue. At 1 month after grafting, only postnatal day 7 grafts contained surviving cells. However, all three types of tissue grafts promoted a unilateral recovery of host dopaminergic fibers on the side of the graft. In striking contrast to the unilateral recovery of the dopaminergic fibers, striatal dopamine levels were increased bilaterally in all tissue grafted mice. These observations suggest that adrenal tissue grafted into the striatum, whether it remains viable or not, has more widespread biochemical effects on the host dopaminergic system than previously recognized. Moreover, these observations bear on mechanisms that may underlie the general recovery of motor disturbances reported in human Parkinson's disease patients who have received a striatal graft of adrenal tissue.
一种帕金森病啮齿动物模型，即经1-甲基-4-(2'-甲基苯基)-1,2,3,6-四氢吡啶处理的小鼠，被用于确定将肾上腺髓质移植到纹状体后纹状体多巴胺水平是否会恢复。进行了四种类型的移植：1) 成年小鼠肾上腺髓质；2) 经冻融以杀死活细胞的成年肾上腺髓质；3) 出生后第7天的肾上腺髓质；4) 无组织的假移植。移植后1个月，只有出生后第7天的移植含有存活细胞。然而，所有三种类型的组织移植均促进了移植侧宿主多巴胺能纤维的单侧恢复。与多巴胺能纤维的单侧恢复形成鲜明对比的是，所有接受组织移植的小鼠纹状体多巴胺水平均双侧升高。这些观察结果表明，移植到纹状体的肾上腺组织，无论其是否存活，对宿主多巴胺能系统具有比先前认识到的更广泛的生化作用。此外，这些观察结果与接受肾上腺组织纹状体移植的人类帕金森病患者所报告的运动障碍总体恢复可能潜在的机制有关。
