Hilton Laura K, White Mark C, Quarmby Lynne M
Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada V5A 1S6.
Biochem Biophys Res Commun. 2009 Nov 6;389(1):52-6. doi: 10.1016/j.bbrc.2009.08.086. Epub 2009 Aug 21.
Mutations in NEK1 in mice are causal for cystic kidneys, and model the ciliopathy polycystic kidney disease caused by abnormal ciliary structure or signaling. NEK1 has previously been shown to localize near centrosomes and to play a role in centrosomal stability and ciliogenesis. Recent data suggest that the etiology of kidney cysts involves aberrant signaling from the primary cilium to the nucleus. Here we demonstrate that NEK1 contains functional nuclear localization signals, is exported from the nucleus via a nuclear export signal-dependent pathway and that the protein cycles through the nucleus. Our data suggest that NEK1 is a candidate to transduce messages from the ciliary-basal body region to the regulation of nuclear gene expression.
小鼠中NEK1的突变是导致肾囊肿的原因，并可模拟由异常纤毛结构或信号传导引起的纤毛病多囊肾病。此前已表明NEK1定位于中心体附近，并在中心体稳定性和纤毛发生中发挥作用。最近的数据表明，肾囊肿的病因涉及从初级纤毛到细胞核的异常信号传导。在这里，我们证明NEK1含有功能性核定位信号，通过核输出信号依赖性途径从细胞核输出，并且该蛋白在细胞核中循环。我们的数据表明，NEK1是将信息从纤毛-基体区域传递到核基因表达调控的候选者。
