Giton Frank, Guéchot Jérôme, Fiet Jean
AP-HP CIB GHU Sud Henri Mondor, Faculté de Médecine, 8 rue du Général Sarrail, Créteil, France.
Clin Biochem. 2009 Nov;42(16-17):1735-8. doi: 10.1016/j.clinbiochem.2009.08.006. Epub 2009 Aug 21.
Preparation of reusable and easy to handle Celite chromatographic columns.
Weighting precise Celite quantities in cartridges and introducing ethylene glycol methanol solutions. The chromatographic solvents pass throughout Celite under negative pressure.
These new minicolumns are reusable. The steroid recoveries' coefficients of variation are less than 10%, and the steroid separation is good.
The reusable Celite cartridge use before steroid immunoassays is easier and less time-consuming than classical glass Celite minicolumns.
制备可重复使用且易于操作的硅藻土色谱柱。
在柱套中精确称取硅藻土并加入乙二醇甲醇溶液。色谱溶剂在负压下通过硅藻土。
这些新型微柱可重复使用。类固醇回收率的变异系数小于10%，类固醇分离效果良好。
在类固醇免疫测定之前使用可重复使用的硅藻土柱套比传统玻璃硅藻土微柱更简便、耗时更少。
