Cytopathology, II Faculty of Medicine, University of Rome La Sapienza, Sant'Andrea Hospital, Rome, Italy.
Cancer Lett. 2010 Mar 1;289(1):40-5. doi: 10.1016/j.canlet.2009.07.017. Epub 2009 Aug 22.
FHIT and WWOX are tumor suppressor genes that span the common fragile sites FRA3B and FRA16D, respectively. To analyze possible synergisms among these genes in cervical cancer progression, we considered 159 cervical intraepithelial neoplasias, and 58 invasive squamous cell carcinomas of the uterine cervix. All cases were previously selected as high risk HPV. FHIT and WWOX proteins were examined by immunohistochemistry and their expression was inversely correlated with precancerous vs. invasive lesions. Statistics among biological markers indicated an association between FHIT and WWOX. Protein expression of these two genes was also absent or reduced in cancer cell lines. Thus, WWOX may be considered as a novel important genetic marker in cervical cancer and the association between the altered expression of FHIT and WWOX may be a critical event in the progression of this neoplasia.
脆性组氨酸三联体（FHIT）和 WW 结构域氧化还原酶 1（WWOX）是分别跨越常见脆弱位点 FRA3B 和 FRA16D 的肿瘤抑制基因。为了分析这两种基因在宫颈癌进展过程中可能存在的协同作用，我们研究了 159 例宫颈上皮内瘤变和 58 例宫颈浸润性鳞状细胞癌。所有病例均被先前选为高危 HPV。通过免疫组织化学检测 FHIT 和 WWOX 蛋白，并将其表达与癌前病变与侵袭性病变进行了比较。生物标志物之间的统计学分析表明 FHIT 和 WWOX 之间存在关联。在癌细胞系中，这两种基因的蛋白表达也缺失或减少。因此，WWOX 可被认为是宫颈癌的一个新的重要遗传标志物，而 FHIT 和 WWOX 表达改变之间的关联可能是这种肿瘤发生发展的一个关键事件。
