Łukasik Iwona, Goławska Sylwia, Leszczyński B
Department of Biochemistry and Molecular Biology, University of Podlasie, ul. B. Prusa 12, 08-110 Siedlce, Poland.
Acta Biol Hung. 2009 Sep;60(3):263-72. doi: 10.1556/ABiol.60.2009.3.3.
Effects of some dietary o-dihydroxyphenols on biochemical markers of oxidative stress within tissues of Sitobion avenae (Fabricius), (Homoptera, Aphididae) and Rhopalosiphum padi (Linnaeus), (Homoptera, Aphididae) has been studied. Among the studied aphid morphs the highest concentration of total thiols, hydrogen peroxide and lipid peroxidation products (TBARS) was noted for winged adults (alatae). Higher content of H2O2 was observed within tissues of the oligophagous species R. padi while the monophagous species S. avenae had higher TBARS and total thiols concentration. Aphids exposed the dietary pro-oxidative o-dihydroxyphenols (quercetin, caffeic acid, chlorogenic acid) demonstrated significantly lower concentration of total sulfhydryls and higher level of TBARS and H2O2 than the control ones. Among the studied compounds, chlorogenic acid showed the strongest effect on the level of thiols and TBARS while caffeic acid caused the highest accumulation of the hydrogen peroxide within the aphid tissues.
研究了一些膳食邻二羟基酚对麦长管蚜（Fabricius）（同翅目，蚜科）和禾谷缢管蚜（Linnaeus）（同翅目，蚜科）组织内氧化应激生化标志物的影响。在所研究的蚜虫形态中，有翅成虫（有翅蚜）的总硫醇、过氧化氢和脂质过氧化产物（硫代巴比妥酸反应物，TBARS）浓度最高。在寡食性物种禾谷缢管蚜的组织中观察到较高的H2O2含量，而单食性物种麦长管蚜的TBARS和总硫醇浓度较高。与对照相比，暴露于膳食促氧化邻二羟基酚（槲皮素、咖啡酸、绿原酸）的蚜虫总巯基浓度显著降低，TBARS和H2O2水平升高。在所研究的化合物中，绿原酸对硫醇和TBARS水平的影响最强，而咖啡酸导致蚜虫组织内过氧化氢积累最多。
