肠炎沙门氏菌肠炎血清型菌毛蛋白SefA在大肠杆菌中作为表面蛋白的克隆赋予了其附着于真核细胞系的能力。
Cloning of Salmonella enterica serovar Enteritidis fimbrial protein SefA as a surface protein in Escherichia coli confers the ability to attach to eukaryotic cell lines.
作者信息
Rank Douglas L, Saeed Mahdi A, Muriana Peter M
机构信息
Department of Food Science, Purdue University, West Lafayette, Indiana 47907, USA.
出版信息
Appl Environ Microbiol. 2009 Oct;75(20):6622-5. doi: 10.1128/AEM.00639-09. Epub 2009 Aug 21.
Abstract
The gene for the Salmonella enterica serovar Enteritidis fimbrial protein SefA was cloned into an Escherichia coli surface expression vector and confirmed by Western blot assay. E. coli clones expressing SefA attached to avian ovary granulosa cells and HEp-2 cells, providing evidence for the involvement of SefA in the ability of Salmonella to attach to eukaryotic cells.
摘要
将肠炎沙门氏菌肠炎血清型菌毛蛋白SefA的基因克隆到大肠杆菌表面表达载体中，并通过蛋白质免疫印迹分析进行了确认。表达SefA的大肠杆菌克隆可附着于禽卵巢颗粒细胞和人喉表皮样癌细胞（HEp-2细胞），这为SefA参与沙门氏菌附着真核细胞的能力提供了证据。
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