Kukkonen M, Raunio T, Virkola R, Lähteenmäki K, Mäkelä P H, Klemm P, Clegg S, Korhonen T K
Department of General Microbiology, University of Helsinki, Finland.
Mol Microbiol. 1993 Jan;7(2):229-37. doi: 10.1111/j.1365-2958.1993.tb01114.x.
Adherence of type-1-fimbriate Salmonella enterica and Escherichia coli to immobilized proteins of the extracellular matrix and reconstituted basement membranes was studied. The type-1-fimbriate strain SH401 of S. enterica serovar Enteritidis showed good adherence to laminin, whereas the adherence to fibronectin, type I, type III, type IV or type V collagens was poor. Only minimal adherence to the matrix proteins was seen with a non-fimbriate strain of S. enterica serovar Typhimurium. A specific and mannoside-inhibitable adhesion to laminin was exhibited by the recombinant E. coli strain HB101(pISF101) possessing fim genes of Typhimurium. Adherence to laminin of strain SH401 was inhibited by Fab fragments against purified SH401 fimbriae, and a specific binding to laminin, of the purified fimbriae, was demonstrated using fimbriae-coated fluorescent microparticles. Periodate treatment of laminin abolished the bacterial adhesion as well as the fimbrial binding. Specific adhesion to immobilized laminin was also shown by the type-1-fimbriate E. coli strain 2131 and the recombinant strain E. coli HB101(pPKL4) expressing the cloned type-1-fimbriae genes of E. coli. Adhesion to laminin of strain HB101(pPKL4) was inhibited by mannoside, and no adherence was seen with the fimH mutant E. coli HB101(pPKL5/pPKL53) lacking the fimbrial lectin subunit. The type-1 fimbriate strains also adhered to reconstituted basement membranes from mouse sarcoma cells and human placenta. Adhesion of strains HB101(pISF101) and HB101(pPKL4) to both basement membrane preparations was inhibited by mannoside. We conclude that type-1 fimbriae of S. enterica and E. coli bind to oligomannoside chains of the laminin network in basement membranes.
研究了1型菌毛的肠炎沙门氏菌和大肠杆菌对细胞外基质固定化蛋白和重组基底膜的黏附情况。肠炎沙门氏菌血清型肠炎菌株SH401的1型菌毛菌株对层粘连蛋白表现出良好的黏附性，而对纤连蛋白、I型、III型、IV型或V型胶原蛋白的黏附性较差。肠炎沙门氏菌血清型鼠伤寒非菌毛菌株对基质蛋白的黏附极少。具有鼠伤寒菌毛基因的重组大肠杆菌菌株HB101(pISF101)对层粘连蛋白表现出特异性且可被甘露糖苷抑制的黏附作用。针对纯化的SH401菌毛的Fab片段可抑制菌株SH401对层粘连蛋白的黏附，并且使用菌毛包被的荧光微粒证明了纯化菌毛与层粘连蛋白的特异性结合。高碘酸盐处理层粘连蛋白可消除细菌黏附以及菌毛结合。1型菌毛的大肠杆菌菌株2131和表达克隆的大肠杆菌1型菌毛基因的重组菌株大肠杆菌HB101(pPKL4)也表现出对固定化层粘连蛋白的特异性黏附。甘露糖苷可抑制菌株HB101(pPKL4)对层粘连蛋白的黏附，而缺乏菌毛凝集素亚基的菌毛H突变体大肠杆菌HB101(pPKL5/pPKL53)则未观察到黏附现象。1型菌毛菌株也黏附于小鼠肉瘤细胞和人胎盘的重组基底膜。甘露糖苷可抑制菌株HB101(pISF101)和HB101(pPKL4)对两种基底膜制剂的黏附。我们得出结论，肠炎沙门氏菌和大肠杆菌的1型菌毛与基底膜中层粘连蛋白网络的低聚甘露糖链结合。
