Berebbi M, Martin P M, Berthois Y, Bernard A M, Blangy D
J.E. CNRS, Marseille, France.
Oncogene. 1990 Apr;5(4):505-9.
We have previously reported (Berebbi et al., 1988) that in athymic nude mice, Polyoma virus induces mammary adenocarcinomas (MAC) at high frequency and exclusively in females. In the present study we show that in nude mice: (1) Ovariectomy results in a reduced frequency of MAC and a longer latency period of induction. When testosterone is administered to ovariectomized females, tumor induction is drastically reduced. (2) When estradiol is administered continuously to ovariectomized females the incidence and kinetics of MAC induction are the same as in control females. (3) MAC are induced in castrated males administered with estradiol although only osteosarcomas are observed in control males. (4) The tumor cells are found to harbor functional estradiol and progesterone receptors. (5) MAC can be transplanted from females to males, indicating that tumor growth is estradiol independent. (6) Estradiol is required only between day 10 and day 20 following polyoma injection, whereas the first tumors are detected only around day 60. Our results indicate that MAC induction by Polyoma virus in nude mice is estradiol-dependent during a short initiation period and that tumor progression is estradiol-independent in spite of the fact tumor cells carry functional estradiol and progesterone receptors.
我们之前报道过（贝雷比等人，1988年），在无胸腺裸鼠中，多瘤病毒能高频诱导乳腺腺癌（MAC），且仅在雌性小鼠中发生。在本研究中，我们发现裸鼠中：（1）卵巢切除导致MAC诱导频率降低，诱导潜伏期延长。当给卵巢切除的雌性小鼠注射睾酮时，肿瘤诱导显著减少。（2）当给卵巢切除的雌性小鼠持续注射雌二醇时，MAC诱导的发生率和动力学与对照雌性小鼠相同。（3）给阉割的雄性小鼠注射雌二醇可诱导MAC，尽管在对照雄性小鼠中仅观察到骨肉瘤。（4）发现肿瘤细胞含有功能性雌二醇和孕酮受体。（5）MAC可从雌性小鼠移植到雄性小鼠，表明肿瘤生长不依赖雌二醇。（6）雌二醇仅在注射多瘤病毒后的第10天至第20天需要，而最早的肿瘤仅在第60天左右被检测到。我们的结果表明，多瘤病毒在裸鼠中诱导MAC在短启动期内依赖雌二醇，尽管肿瘤细胞携带功能性雌二醇和孕酮受体，但肿瘤进展不依赖雌二醇。
