原发性结直肠癌及其肝转移灶中表皮生长因子受体（EGFR）mRNA 水平和蛋白表达水平。
Epidermal growth factor receptor (EGFR) mRNA levels and protein expression levels in primary colorectal cancer and corresponding liver metastases.
机构信息
Department of Gastroenterological Surgery, Tokyo Women's Medical University, 8-1 Kawadacho, Shinjukuku, Tokyo 162-8666, Japan.
出版信息
Cancer Chemother Pharmacol. 2010 Apr;65(5):825-31. doi: 10.1007/s00280-009-1087-5. Epub 2009 Aug 22.
PURPOSE
High expression levels of EGFR mRNA are reported to be associated with a higher response probability in epidermal growth factor receptor (EGFR) targeted drugs. Our aim was to determine how EGFR gene expression levels in primary colorectal cancer (CRC) were related to those in liver metastases.
METHODS
31 pairs of primary CRC and corresponding liver metastases were analyzed. Gene expression level was measured using real-time RT-PCR.
RESULTS
No significant difference was observed between median mRNA expression levels of EGFR in primary cancer and those in corresponding liver metastases (P = 0.99). When matched tissue sets were compared on an individual basis, there was a significant correlation for EGFR mRNA expression between primary cancer and corresponding liver metastases (rs = 0.78, P < 0.0001).
CONCLUSIONS
A good prediction of EGFR mRNA levels in liver metastases can be obtained by measuring those in the primary CRC.
目的
据报道，表皮生长因子受体（EGFR）mRNA 表达水平高与 EGFR 靶向药物的更高反应概率相关。我们的目的是确定原发性结直肠癌（CRC）中 EGFR 基因表达水平与肝转移中的表达水平有何关系。
方法
分析了 31 对原发性 CRC 和相应的肝转移瘤。使用实时 RT-PCR 测量基因表达水平。
结果
原发性癌症和相应肝转移瘤中 EGFR mRNA 表达水平的中位数无显著差异（P = 0.99）。当在个体基础上比较匹配的组织时，原发性癌症和相应肝转移瘤之间 EGFR mRNA 表达存在显著相关性（rs = 0.78，P < 0.0001）。
结论
通过测量原发性 CRC 中的 EGFR mRNA 水平，可以很好地预测肝转移瘤中的 EGFR mRNA 水平。
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