Sato Tsutomu, Oshima Takashi, Yoshihara Kazue, Yamamoto Naoto, Yamada Roppei, Nagano Yasuhiko, Fujii Shoichi, Kunisaki Chikara, Shiozawa Manabu, Akaike Makoto, Rino Yasushi, Tanaka Katsuaki, Masuda Munetaka, Imada Toshio
Gastroenterological Center, Yokohama City University Medical Center, Kanagawa-ken 232-0024, Japan.
Oncol Rep. 2009 Jan;21(1):211-6.
Expression of the fibroblast growth factor (FGF)-1, FGF-2, fibroblast growth factor receptor (FGFR)-1, and FGFR-2 genes has been reported in various cancers and is associated with poor outcomes in patients with solid tumors. This study examined the relations between the relative expression of the FGF genes and clinicopathological factors, especially invasion and metastasis, in patients with colorectal cancer. We studied surgical specimens of cancer tissue and adjacent normal mucosa obtained from 202 patients with untreated colorectal carcinoma. The relative expression levels of FGF-1, FGF-2, FGFR-1, and FGFR-2 mRNA in cancer and in normal adjacent mucosa were measured by quantitative real-time, reverse-transcription polymerase chain reaction. The relative expression level of the FGFR-2 gene was higher in normal adjacent mucosa than in cancer, whereas the relative expression levels of the FGF-1, FGF-2, and FGFR-1 genes were similar. FGFR-1 gene expression levels were higher in the presence than in the absence of liver metastasis. An analysis of the relation between clinicopathological features and gene expression showed that overexpression of FGFR-1 correlated with liver metastasis. Our results suggested that overexpression of the FGFR-1 gene might lead to liver metastasis in colorectal cancer. Overexpression of the FGFR-1 gene may thus be a useful predictor of liver metastasis in patients with colorectal cancer.
成纤维细胞生长因子（FGF）-1、FGF-2、成纤维细胞生长因子受体（FGFR）-1和FGFR-2基因的表达已在多种癌症中被报道，并且与实体瘤患者的不良预后相关。本研究检测了FGF基因的相对表达与结直肠癌患者临床病理因素之间的关系，尤其是侵袭和转移。我们研究了从202例未经治疗的结直肠癌患者获取的癌组织手术标本和相邻正常黏膜。通过定量实时逆转录聚合酶链反应测量癌组织和相邻正常黏膜中FGF-1、FGF-2、FGFR-1和FGFR-2 mRNA的相对表达水平。FGFR-2基因在相邻正常黏膜中的相对表达水平高于癌组织，而FGF-1、FGF-2和FGFR-1基因的相对表达水平相似。存在肝转移时FGFR-1基因表达水平高于无肝转移时。临床病理特征与基因表达关系的分析表明，FGFR-1过表达与肝转移相关。我们的结果提示，FGFR-1基因过表达可能导致结直肠癌肝转移。因此，FGFR-1基因过表达可能是结直肠癌患者肝转移的一个有用预测指标。
