Department of Biotechnology and Bioengineering CINVESTAV-IPN, Av. IPN 2508, México, DF, Mexico.
Arch Microbiol. 2009 Oct;191(10):745-50. doi: 10.1007/s00203-009-0502-y. Epub 2009 Aug 23.
Cellulomonas flavigena produces a battery of cellulase components that act concertedly to degrade cellulose. The addition of cAMP to repressed C. flavigena cultures released catabolic repression, while addition of cAMP to induced C. flavigena cultures led to a cellobiohydrolase hyperproduction. Exogenous cAMP showed positive regulation on cellobiohydrolase production in C. flavigena grown on sugar cane bagasse. A C. flavigena cellobiohydrolase gene was cloned (named celA), which coded for a 71- kDa enzyme. Upstream, a repressor celR1, identified as a 38 kDa protein, was monitored by use of polyclonal antibodies.
黄色噬纤维菌能产生一系列协同作用的纤维素酶组件，用于降解纤维素。向受抑制的黄色噬纤维菌培养物中添加 cAMP 可以解除分解代谢抑制，而向诱导的黄色噬纤维菌培养物中添加 cAMP 则导致细胞外切酶 hyperproduction。外源性 cAMP 对在甘蔗渣上生长的黄色噬纤维菌的细胞外切酶生产显示出正调节作用。克隆了黄色噬纤维菌的一个细胞外切酶基因（命名为 celA），该基因编码一个 71-kDa 的酶。在其上游，使用多克隆抗体监测到一个 38-kDa 的阻遏物 celR1。
