慢性丙型肝炎病毒感染中的内毒素受体CD14基因变体与组织学特征

Endotoxin receptor CD14 gene variants and histological features in chronic HCV infection.

作者信息

Askar Eva, Ramadori Giuliano, Mihm Sabine

机构信息

Department of Gastroenterology and Endocrinology, University Medical Center, Georg-August-University, Robert-Koch-Strasse 40, D-37075 Goettingen, Germany.

出版信息

World J Gastroenterol. 2009 Aug 21;15(31):3884-90. doi: 10.3748/wjg.15.3884.

DOI:10.3748/wjg.15.3884

PMID:19701968

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2731250/

Abstract

AIM

To analyze the correlation between CD14 rs2569190/C-159T single nucleotide polymorphism (SNP) and disease progression in chronic hepatitis C.

METHODS

Liver biopsy specimens from a total of 137 and 349 patients with chronic hepatitis C were separately evaluated with respect to necroinflammatory activity (grading) and architectural changes (staging). In one group, further histological lesions characteristic for hepatitis C, hepatitis C virus subtypes, and biochemical parameters of liver disease were also investigated. Samples of genomic DNA were genotyped for the respective SNP by 5'-nuclease assays using fluorescent dye-labeled allele-specific probes.

RESULTS

Genotype distribution did not deviate from the Hardy-Weinberg equilibrium. In the first group, patients homozygous for the variant allele T were found to be younger than C allele carriers (39.6 +/- 12.5 vs 45.7 +/- 11.5, P = 0.008). Among the histological lesions studied, portal lymphoid aggregates were more frequently observed among TT homozygotes than among C carriers (21/37 vs 32/100, P = 0.008). The presence of portal lymphoid aggregates was closely correlated with hepatic inflammation (P = 0.003) and with bile duct damage (P < 0.001). The degree of fibrosis, in contrast, was not found to be related to the CD14 gene C-159T polymorphism.

CONCLUSION

The data suggest a possible relationship between CD14 C-159T polymorphism and the formation of portal lymphoid aggregates, but not liver fibrosis progression in chronic hepatitis C.

摘要

目的

分析CD14 rs2569190/C-159T单核苷酸多态性（SNP）与慢性丙型肝炎疾病进展之间的相关性。

方法

分别对总共137例和349例慢性丙型肝炎患者的肝活检标本进行坏死性炎症活动（分级）和结构改变（分期）评估。在一组患者中，还研究了丙型肝炎特有的进一步组织学病变、丙型肝炎病毒亚型以及肝病的生化参数。使用荧光染料标记的等位基因特异性探针，通过5'-核酸酶测定法对基因组DNA样本进行相应SNP的基因分型。

结果

基因型分布未偏离哈迪-温伯格平衡。在第一组中，发现变异等位基因T的纯合患者比C等位基因携带者更年轻（39.6±12.5对45.7±11.5，P = 0.008）。在所研究的组织学病变中，TT纯合子中门静脉淋巴滤泡的观察频率高于C等位基因携带者（21/37对32/100，P = 0.008）。门静脉淋巴滤泡的存在与肝脏炎症密切相关（P = 0.003），也与胆管损伤密切相关（P < 0.001）。相比之下，纤维化程度与CD14基因C-159T多态性无关。