Henriques Isabel S, Almeida Adelaide, Cunha Angela, Correia António
Departamento de Biologia, Centro de Biologia Celular, Campus Universitário de Santiago, Universidade de Aveiro, 3810-193 Aveiro, Portugal.
FEMS Microbiol Ecol. 2004 Aug 1;49(2):269-79. doi: 10.1016/j.femsec.2004.04.003.
Construction of 16S rDNA libraries was undertaken to examine the structure of free-living bacterial communities in the estuarine system Ria de Aveiro, Portugal. Samples were collected in April 2002, from two sites representing marine (station N1) and brackish (station I6) water zones. Clones were characterized by RFLP patterns and sequence analysis of representative clones revealed that both libraries were clearly dominated by alpha-proteobacteria, followed by gamma-proteobacteria and beta-proteobacteria. Clones affiliated with the delta-proteobacteria, Verrucomicrobia, Bacteroidetes and Actinobacteria were exclusive of station I6 and sequences related to the Firmicutes were only found in station N1. Sequences retrieved are included in only a few major bacterial divisions and in general, shared a high degree of homology with sequences deposited in nucleotide databases, and recovered from aquatic environments of diverse geographic regions. Differences between the two sites may reflect adaptation to different environmental conditions, especially salinity. The pattern of prokaryotic diversity is comparable to other coastal and estuarine environments previously studied.
构建16S rDNA文库以研究葡萄牙阿威罗河口系统中自由生活细菌群落的结构。2002年4月从代表海洋（N1站）和咸淡水（I6站）水域的两个地点采集样本。通过RFLP模式对克隆进行表征，对代表性克隆的序列分析表明，两个文库均明显以α-变形菌为主，其次是γ-变形菌和β-变形菌。与δ-变形菌、疣微菌门、拟杆菌门和放线菌门相关的克隆仅在I6站存在，与厚壁菌门相关的序列仅在N1站发现。检索到的序列仅包含在少数主要细菌类群中，总体而言，与核苷酸数据库中 deposited的序列具有高度同源性，并且是从不同地理区域的水生环境中 recovered的。两个地点之间的差异可能反映了对不同环境条件的适应，尤其是盐度。原核生物多样性模式与先前研究的其他沿海和河口环境 comparable。
