Willshaw G A, McConnell M M, Smith H R, Rowe B
Division of Enteric Pathogens, Central Public Health Laboratory, London, U.K.
FEMS Microbiol Lett. 1990 Mar 15;56(3):255-60.
Two enterotoxigenic Escherichia coli strains of serotype 0.25.H42 that produced coli surface associated antigens CS4 and CS6 hybridized with a probe containing the cfaD sequence that regulates expression of colonization factor antigen CFA/I. Transformation of a cloned cfaD gene into some derivatives of the strains that were negative for CS4 and CS6 resulted in expression of CS4 but not CS6. By hybridization the sequence that regulated CS4 production in the wild type 025 strains was located on a plasmid that also encoded the CS6 antigen. The structural genes for the CS4 antigen were on a separate plasmid. The 025 strains carried a third plasmid encoding enterotoxin production which was therefore unlinked to regulation sequences or genes encoding CS antigens.
两株血清型为0.25.H42的产肠毒素大肠杆菌菌株产生了与菌毛表面相关的抗原CS4和CS6，它们与一个包含调控定居因子抗原CFA/I表达的cfaD序列的探针杂交。将克隆的cfaD基因导入CS4和CS6呈阴性的部分菌株衍生物中，结果导致CS4表达，但CS6未表达。通过杂交发现，野生型025菌株中调控CS4产生的序列位于一个也编码CS6抗原的质粒上。CS4抗原的结构基因位于一个单独的质粒上。025菌株携带第三个编码肠毒素产生的质粒，因此该质粒与编码CS抗原的调控序列或基因没有联系。
