Wei Yuanlong, Lan Tao, Tang Tao, Zhang Lingyi, Wang Fengyun, Li Tong, Du Yiping, Zhang Weibing
School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
J Chromatogr A. 2009 Oct 30;1216(44):7466-71. doi: 10.1016/j.chroma.2009.08.029. Epub 2009 Aug 14.
A comprehensive orthogonal two-dimensional liquid chromatography (2D-LC) based on the modification of mobile phases was developed with a sample loop-valve interface. To improve the compatibility of mobile phases and analysis speed, some special solvents were chosen as the mobile phases, and the column temperature was elevated to decrease the viscosity of mobile phases of reversed-phase liquid chromatography (RPLC). Based on this principle, the first dimension was normal-phase liquid chromatography (NPLC) with a SiO2 column, and the second dimension was reversed-phase liquid chromatography containing two tandem C18 columns. 1,4-Dioxane was used in the NPLC mobile phase, and isopropyl alcohol was employed in the RPLC mobile phase. Moreover, the elevated column temperature enabled the reduction of the backpressure and using tandem C18 columns to improve the resolving power in RPLC. The new comprehensive 2D-LC system and applied strategy offered a novel idea for construction of 2D-LC system. A traditional Chinese medicine, Zhengtian pill, was used as the test sample to evaluate the constructed 2D-LC system. 876 peaks were detected, and the peak capacity reached 1740.
基于流动相改性的综合正交二维液相色谱（2D-LC）结合进样环阀接口得以开发。为提高流动相兼容性和分析速度，选择了一些特殊溶剂作为流动相，并提高柱温以降低反相液相色谱（RPLC）流动相的粘度。基于此原理，第一维采用SiO₂ 柱进行正相液相色谱（NPLC），第二维采用含两根串联C18柱的反相液相色谱。NPLC流动相中使用1,4-二氧六环，RPLC流动相中使用异丙醇。此外，柱温升高可降低背压，并使用串联C18柱提高RPLC的分离能力。新的综合二维液相色谱系统及应用策略为二维液相色谱系统的构建提供了新思路。以中药正天丸作为测试样品对构建的二维液相色谱系统进行评价。共检测到876个峰，峰容量达到1740。
