Yang Liang, Xu Bing, Yuan Chunxu, Dai Zhi, Wang Yong, Li Qiongya, Yang Qifan, Li Nuomin, Qing Hong
School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
College of Life Sciences & Research Center for Resource Peptide Drugs, Shaanxi Engineering & Technological Research Center for Conversation & Utilization of Regional Biological Resources, Yanan University, Yanan 716000, China.
Evid Based Complement Alternat Med. 2019 Mar 10;2019:5421528. doi: 10.1155/2019/5421528. eCollection 2019.
So far, more than 25,000 brain diseases have been shown to be related to oxidative stress. Excessive free radicals and reactive oxygen species (ROS) can attack cells resulting in dysfunctional proteins, lipids, and nucleic acid, finally leading to imbalance of energy metabolism, cell death, gene mutation, and immune reaction. Therefore oxidative stress plays an important role in neuronal diseases. As a traditional Chinese medicine, Zhengtian Pill (ZTP) was reported to have the ability to reduce the blood viscosity of migraine model rats, with increased beta-endorphin, serotonin, adrenaline, and dopamine in brain tissue. Moreover ZTP can effectively accelerate blood circulation and attenuate blood coagulation. However, the molecular mechanisms of ZPT are still unclear. Through the behavioral test we found that ZTP can significantly improve depression-like behavior induced by LPS when rat was treated with ZTP (L 0.17 g/kg, M 0.34 g/kg, and H 0.7 g/kg) intraperitoneal injection once a day for 30 consecutive days. And ZTP can resist oxidative stress (>72 h) for a longer time. And ZTP can promote the levels of ATP and SOD and reduce the levels of ROS and MDA in the brain. At the same time, ZTP can have antioxidant stress through increasing the expression level of Nrf2/HO-1/P38. These results show that ZTP may be a potential antioxidant stress drug for variety of diseases associated with oxidative stress injury.
到目前为止，已证实超过25000种脑部疾病与氧化应激有关。过量的自由基和活性氧（ROS）会攻击细胞，导致蛋白质、脂质和核酸功能失调，最终导致能量代谢失衡、细胞死亡、基因突变和免疫反应。因此，氧化应激在神经元疾病中起着重要作用。正天丸（ZTP）作为一种中药，据报道具有降低偏头痛模型大鼠血液粘度的能力，可使脑组织中的β-内啡肽、血清素、肾上腺素和多巴胺增加。此外，正天丸能有效促进血液循环并减弱血液凝固。然而，正天丸的分子机制仍不清楚。通过行为测试，我们发现当大鼠连续30天每天腹腔注射正天丸（低剂量0.17 g/kg、中剂量0.34 g/kg、高剂量0.7 g/kg）时，正天丸可显著改善由脂多糖诱导的抑郁样行为。而且正天丸能在较长时间（>72小时）内抵抗氧化应激。并且正天丸可提高大脑中ATP和SOD的水平，降低ROS和MDA的水平。同时，正天丸可通过增加Nrf2/HO-1/P38的表达水平产生抗氧化应激作用。这些结果表明，正天丸可能是一种针对多种与氧化应激损伤相关疾病的潜在抗氧化应激药物。
