用免疫电子显微镜法确定百日咳博德特氏菌三种主要凝集原的位置

Location of the three major agglutinogens of Bordetella pertussis by immuno-electronmicroscopy.

作者信息

Preston N W, Zorgani A A, Carter E J

机构信息

Pertussis Reference Laboratory, Department of Medical Microbiology, University Medical School, Manchester.

出版信息

J Med Microbiol. 1990 May;32(1):63-8. doi: 10.1099/00222615-32-1-63.

DOI:10.1099/00222615-32-1-63

PMID:1971311

Abstract

When the three serotypes of Bordetella pertussis (types 1,2,3; 1,2 and 1,3) were labelled with agglutinins and protein-A gold, agglutinogen 1 was found on fimbriae and on the cell surface of types 1,2,3 and 1,2 but on the cell surface only of non-fimbriate type 1,3 organisms. In contrast, agglutinogen 2 was located on fimbriae only. Agglutinogen 3 was not labelled. When protein-A gold was replaced by immunoglobulin-G gold, agglutinogen 3 was found on the cell surface only, even of fimbriate bacteria of type 1,2,3. The implications of these findings for acellular vaccines are discussed.

摘要

当用凝集素和蛋白A金标记百日咳博德特氏菌的三种血清型（1、2、3型；1、2型和1、3型）时，在1、2、3型和1、2型的菌毛及细胞表面发现了凝集原1，但仅在无菌毛的1、3型菌株的细胞表面发现了凝集原1。相比之下，凝集原2仅位于菌毛上。凝集原3未被标记。当用免疫球蛋白G金取代蛋白A金时，即使是1、2、3型有菌毛的细菌，凝集原3也仅在细胞表面被发现。本文讨论了这些发现对无细胞疫苗的意义。