Lee In-Kyoung, Seo Geon-Sik, Jeon Nak Beom, Kang Hee-Wan, Yun Bong-Sik
Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk, Korea.
J Antibiot (Tokyo). 2009 Nov;62(11):631-4. doi: 10.1038/ja.2009.82. Epub 2009 Aug 28.
Novel styrylpyrones, phellinins A1 and A2, were isolated together with known styrylpyrone compounds, hispidin and 1,1-distyrylpyrylethan, from the cultured broth of Phellinus sp. KACC93057P. These compounds were purified by solvent partition, Sephadex LH-20 column chromatography, C(18)-solid phase extraction and finally by reversed-phase (ODS) TLC. To identify the phellinin producer Phellinus sp. KACC93057P, the ribosomal DNA (rDNA) internal transcribed space regions containing 5.8 rDNA were sequenced and compared with those of the known Phellinus isolates. Phellinus sp. KACC93057P was 94.8% identical to P. baumii and P. linteus, all of which did not produce phellinins A1 and A2. These compounds significantly scavenged free radicals such as 1,1-diphenyl-2-picrylhydrazyl, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) and superoxide.
从桑黄属菌株Phellinus sp. KACC93057P的培养液中分离出了新型苯乙烯基吡喃酮类化合物桑黄菌素A1和A2，同时还分离出了已知的苯乙烯基吡喃酮类化合物漆斑菌素和1,1-二苯乙烯基吡喃基乙烷。这些化合物通过溶剂分配、葡聚糖凝胶LH-20柱色谱、C(18)固相萃取，最后通过反相（ODS）薄层色谱进行纯化。为了鉴定桑黄菌素的产生菌Phellinus sp. KACC93057P，对包含5.8 rDNA的核糖体DNA（rDNA）内部转录间隔区进行了测序，并与已知的桑黄属分离株进行了比较。Phellinus sp. KACC93057P与鲍氏桑黄和皱盖乌芝的相似度为94.8%，而这两种菌均不产生桑黄菌素A1和A2。这些化合物能有效清除1,1-二苯基-2-苦基肼、2,2'-偶氮二异丁基脒二盐酸盐和超氧阴离子等自由基。
