Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan.
Department of Oral Medicine, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan.
Int J Syst Evol Microbiol. 2010 Jul;60(Pt 7):1637-1639. doi: 10.1099/ijs.0.017020-0. Epub 2009 Aug 28.
Four strains (NUM 1903(T), NUM 1904, NUM 1912 and NUM 1925) that were obligately anaerobic, pigmented, Gram-negative-staining rods were isolated from the oral cavity of donkeys. These strains were analysed using the Rapid ID 32A, API 20A and API ZYM systems, by DNA-DNA hybridization with other related species and by 16S rRNA gene sequencing. 16S rRNA gene sequence analysis showed that each of the new isolates was a member of the genus Prevotella and related to Prevotella multiformis PPPA21(T), showing about 93 % sequence similarity. Based on phylogenetic and phenotypic evidence, it is proposed that the four strains are representatives of a novel species, for which the name Prevotella dentasini sp. nov. is proposed. The type strain is NUM 1903(T) (=JCM 15908(T)=DSM 22229(T)).
从驴口腔中分离到四株严格厌氧、色素、革兰氏阴性染色的杆菌(NUM 1903(T)、NUM 1904、NUM 1912 和 NUM 1925)。使用 Rapid ID 32A、API 20A 和 API ZYM 系统、与其他相关物种的 DNA-DNA 杂交以及 16S rRNA 基因测序对这些菌株进行了分析。16S rRNA 基因序列分析表明,每个新分离株均为普雷沃氏菌属的成员,与多形拟杆菌 PPPA21(T) 具有约 93%的序列相似性。基于系统发育和表型证据,建议这四株菌代表一个新的种,提议将其命名为牙普雷沃氏菌(Prevotella dentasini)sp. nov.。模式菌株为 NUM 1903(T)(=JCM 15908(T)=DSM 22229(T))。