用于高灵敏度检测嗜热菌蛋白酶的镧系元素荧光肽底物。
Lanthanide-based fluorogenic peptide substrate for the highly sensitive detection of thermolysin.
作者信息
Marguerre Ann-Kathrin, Krämer Roland
机构信息
Anorganisch-Chemisches Institut, Ruprecht-Karls-Universität Heidelberg, Heidelberg, Germany.
出版信息
Bioorg Med Chem Lett. 2009 Oct 1;19(19):5757-9. doi: 10.1016/j.bmcl.2009.07.152. Epub 2009 Aug 4.
PMID:19720529
Abstract
A new fluorogenic, lanthanide-based oligopeptide substrate for the detection of the zinc-dependent endoprotease thermolysin is described. Using time-resolved fluorescence measurement, a highly sensitive assay for thermolysin was developed with a 50pM detection limit (3.5fmol).
摘要
本文描述了一种用于检测锌依赖性内肽酶嗜热菌蛋白酶的新型基于镧系元素的荧光寡肽底物。利用时间分辨荧光测量技术，开发了一种对嗜热菌蛋白酶高度灵敏的检测方法，检测限为50pM（3.5fmol）。
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