印度南部金奈注射吸毒者中主要的HIV-1耐药性及多态性模式

Primary HIV-1 drug resistance and polymorphic patterns among injecting drug users (IDUs) in Chennai, Southern India.

作者信息

Iqbal H Syed, Solomon Sunil S, Madhavan Vidya, Solomon Suniti, Balakrishnan P

机构信息

YRG Centre for AIDS Research and Education (YRG CARE), Voluntary Health Services Hospital Campus, Taramani, Chennai, India.

出版信息

J Int Assoc Physicians AIDS Care (Chic). 2009 Sep-Oct;8(5):323-7. doi: 10.1177/1545109709341854. Epub 2009 Aug 31.

DOI:10.1177/1545109709341854

PMID:19721100

Abstract

BACKGROUND AND OBJECTIVE

India has 1.1 million injecting drug users (IDUs) with an HIV prevalence rate as high as 64%. Drug resistance screening before therapy is beneficial to the individual. Here, we have studied primary drug resistance among IDUs in Chennai.

METHODS

Specimens (n = 55) collected between March 2005 and April 2006 were subjected to genotyping assay. The mutations for the drug resistance were interpreted using the Stanford HIV Drug Resistance Database.

RESULTS

M41LM (1.8%), K65KN (1.8%), and G73GS (2.7%) were found to be associated with low-level resistance to zidovudine (ZDV), stavudine (d4T), abacavir (ABC), didanosine (ddI), emtricitabine (FTC), tenofovir (TDF), and saquinavir (SQV) in each specimen. The rate of polymorphisms is significantly different from universally established subtype ''C''-specific polymorphisms (P < .0001).

CONCLUSION

The presence of drug resistance mutations, though minimal, is alarming as it could spread easily between IDUs and from them to their sexual partners. The genetic variation is of importance in vaccine design.

摘要

背景与目的

印度有110万注射吸毒者，其艾滋病毒感染率高达64%。治疗前进行耐药性筛查对个体有益。在此，我们研究了钦奈注射吸毒者中的原发性耐药情况。

方法

对2005年3月至2006年4月间收集的55份标本进行基因分型检测。使用斯坦福艾滋病毒耐药数据库解读耐药性突变情况。

结果

在每份标本中，发现M41LM（1.8%）、K65KN（1.8%）和G73GS（2.7%）与对齐多夫定（ZDV）、司他夫定（d4T）、阿巴卡韦（ABC）、去羟肌苷（ddI）、恩曲他滨（FTC）、替诺福韦（TDF）和沙奎那韦（SQV）的低水平耐药有关。多态性发生率与普遍确定的C亚型特异性多态性有显著差异（P <.0001）。