Hajipour B, Somi M H, Saberifar F, Hemmati M R, Asl N A, Moein A, Vatankhah A M, Nourazar A R, Nasirizade M R
Faculty of Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran.
Folia Morphol (Warsz). 2009 Aug;68(3):156-62.
Ischaemia/reperfusion (I/R) injury is commonly seen in the field of intestine surgical interventions, shock, trauma, and many other clinical conditions. Simvastatin is known to have antioxidant and anti-inflammatory properties. This study investigated the effect of simvastatin administration in a warm intestinal I/R model on TNF-alpha, antioxidant enzymes and intestinal tissue morphology. Thirty-six male wistar rats underwent laparotomy under general anaesthesia. Simvastatin was administered from four days before ischaemia induction. The rats were divided in to three groups (n = 12): the sham group, the I/R group, and the I/R + simvastatin group. Intestinal ischaemia was induced by superior mesenteric artery ligation with microvascular clamps for 60 minutes, and after ischaemia, blood perfusion was released into the tissue and a reperfusion phase was started, which lasted for 3 hours. After 3 hours, the animals were sacrificed and serum and tissue obtained for biochemical and histological study. In the simvastatin treated group, intestinal tissue injury, TNF-alpha level, and tissue malondealdehyde levels were significantly lower than in the I/R group (p < 0.05). Glutathion peroxidase and superoxide dismutase levels were significantly higher in the simvastatin treated group than in the I/R group (p < 0.05). Simvastatin pretreatment reduced intestinal I/R injury and was associated with down- -regulation of serum TNF-alpha and tissue malondealdehyde level, and simvastatin administration maintained cellular antioxidant enzyme contents compared to the I/R group after 3 hours reperfusion time.
缺血/再灌注（I/R）损伤在肠道手术干预、休克、创伤及许多其他临床病症领域中较为常见。已知辛伐他汀具有抗氧化和抗炎特性。本研究调查了在温暖肠道I/R模型中给予辛伐他汀对肿瘤坏死因子-α（TNF-α）、抗氧化酶及肠道组织形态的影响。36只雄性Wistar大鼠在全身麻醉下接受剖腹手术。在诱导缺血前四天给予辛伐他汀。大鼠被分为三组（n = 12）：假手术组、I/R组和I/R +辛伐他汀组。通过用微血管夹结扎肠系膜上动脉60分钟诱导肠道缺血，缺血后，血液灌注释放到组织中并开始再灌注阶段，持续3小时。3小时后，处死动物并获取血清和组织用于生化和组织学研究。在辛伐他汀治疗组中，肠道组织损伤、TNF-α水平和组织丙二醛水平显著低于I/R组（p < 0.05）。辛伐他汀治疗组中的谷胱甘肽过氧化物酶和超氧化物歧化酶水平显著高于I/R组（p < 0.05）。辛伐他汀预处理减轻了肠道I/R损伤，且与血清TNF-α和组织丙二醛水平的下调相关，并且与I/R组相比，在再灌注3小时后给予辛伐他汀可维持细胞抗氧化酶含量。
