Department of Biological and Environmental Sciences, University of Sannio, Via Port'Arsa, 11, Benevento 82100, Italy.
Microsc Res Tech. 2010 Mar;73(3):206-14. doi: 10.1002/jemt.20776.
A progestin receptor (PR) has been detected in the olfactory organ of the trout Salmo trutta fario. The specificity of this receptor was high for 17alpha,20beta-dihydroxy-4-pregnen-3-one (17alpha,20beta-DP), but it also bound 17alpha-hydroxy-progesterone (17alpha-OHP) and 21-hydroxyprogesterone (21-OHP), even when present at low concentrations (10-fold in relative binding affinity assay). Progesterone (P) competed effectively at much higher concentrations (1,000-fold in relative binding affinity assay). Immunohistochemical studies carried out with three different monoclonal antibodies against human progesterone receptor (hPR), chicken progesterone receptor hinge region (cPR), and chicken progesterone receptor A/B domain (PR22), revealed that immunoreactivity was present in the epithelium of the olfactory organ of females and males of the trout Salmo trutta fario only against hPR. Western blotting showed two hPR immunoreactive bands of about 62 and 66 kDa. Finally, a portion of the cDNA of about 300 nucleotides extending over the DNA binding domain and the ligand binding domain was cloned and sequenced, revealing a high degree of sequence homology of the PR in Salmo trutta fario with the PR in other teleosts.
在鳟鱼的嗅觉器官中已经检测到孕激素受体（PR）。该受体对 17α，20β-二羟-4-孕烯-3-酮（17α，20β-DP）具有很高的特异性，但它也能结合 17α-羟孕酮（17α-OHP）和 21-羟孕酮（21-OHP），即使在低浓度下（相对结合亲和力测定中为 10 倍）也是如此。孕酮（P）在更高浓度下（相对结合亲和力测定中为 1000 倍）能有效地竞争。用针对人孕激素受体（hPR）、鸡孕激素受体铰链区（cPR）和鸡孕激素受体 A/B 结构域（PR22）的三种不同单克隆抗体进行的免疫组织化学研究表明，只有 hPR 对雌性和雄性鳟鱼的嗅觉器官上皮具有免疫反应性。Western blot 显示有两个约 62 和 66 kDa 的 hPR 免疫反应性带。最后，克隆并测序了约 300 个核苷酸的 cDNA，该 cDNA 延伸到 DNA 结合域和配体结合域，揭示了鳟鱼 PR 与其他硬骨鱼 PR 的高度序列同源性。
