Carson Connor, Quinnell Rupert J, Day Michael J, Courtenay Orin
Populations and Disease Research Group, Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
Vet Immunol Immunopathol. 2010 Feb 15;133(2-4):264-8. doi: 10.1016/j.vetimm.2009.07.017. Epub 2009 Aug 7.
In murine models of leishmaniasis, IgG subclass expression is a proxy measure for Th1/Th2 cellular immune response bias. However, in dogs, the reservoir of zoonotic visceral leishmaniasis, no consistent association has been described between IgG subclass ratios and disease resistance. Inconsistent results may reflect lack of specificity of commonly used commercial antibodies. Our aim was to measure IgG1 and IgG2 responses to crude Leishmania antigen using commercial polyclonal antibodies for comparison with a panel of commercially unavailable monoclonal antibodies, in a cohort of 60 naturally infected dogs, and to compare associations between subclass responses and clinical or parasitological outcomes. IgG1 and IgG2, measured by both antibodies, were higher in clinically symptomatic than in asymptomatic dogs (P</=0.03), reflecting general upregulation of IgG in infected dogs. Unlike the murine model, canine IgG2:IgG1 ratios were not predictive of clinical or parasitological outcomes of infection. Associations between subclass levels and positivity by bone marrow culture and PCR were not consistent when measured with different antibodies. Further research is needed to re-evaluate the specificity of commercially available IgG subclass antibodies.
在利什曼病的小鼠模型中，IgG亚类表达是Th1/Th2细胞免疫反应偏向性的一种替代指标。然而，在作为人兽共患内脏利什曼病宿主的犬类中，IgG亚类比例与抗病性之间尚未发现一致的关联。结果不一致可能反映了常用商业抗体缺乏特异性。我们的目的是在60只自然感染犬组成的队列中，使用商业多克隆抗体测量对粗制利什曼原虫抗原的IgG1和IgG2反应，并与一组无法通过商业途径获得的单克隆抗体进行比较，同时比较亚类反应与临床或寄生虫学结果之间的关联。两种抗体测量的IgG1和IgG2在有临床症状的犬中高于无症状犬（P≤0.03），这反映了感染犬中IgG的普遍上调。与小鼠模型不同，犬的IgG2:IgG1比例不能预测感染的临床或寄生虫学结果。当用不同抗体测量时，亚类水平与骨髓培养和PCR阳性之间的关联并不一致。需要进一步研究重新评估市售IgG亚类抗体的特异性。
