Iniesta Laura, Gállego Montserrat, Portús Montserrat
Laboratori de Parasitologia, Facultat de Farmàcia, Universitat de Barcelona, Avda, Joan XXIII s/n, E-08028 Barcelona, Spain.
Vet Immunol Immunopathol. 2007 Oct 15;119(3-4):189-97. doi: 10.1016/j.vetimm.2007.05.006. Epub 2007 Jun 2.
Here we analyzed, by Western blot analysis, the idiotype expression of IgG1 and IgG2 in 109 canine sera corresponding to 50 dogs from endemic areas of leishmaniosis in order to detect markers related to Leishmania infantum infection and clinical condition (asymptomatic or symptomatic). Twenty-four dogs from an area free of leishmaniosis were used as controls. IgG1 and IgG2 responses in symptomatic and asymptomatic L. infantum infections differed mainly in subclass production (ELISA values), with higher IgG2 production occurring particularly in symptomatic dogs. Nevertheless, we observed little difference in the idiotype expression of these IgG subclasses, which, in general, recognized the same antigenic fractions. While early L. infantum infection was characterized by recognition of polypeptide fractions of low molecular weight, mainly fractions of 14, 16 and 18 kDa by IgG1 and 14 and 16 kDa by IgG2, symptomatology was associated with recognition by both IgG subclasses of a 24 kDa fraction and other antigens belonging to the AG24 family.
在此，我们通过蛋白质免疫印迹分析，检测了来自利什曼病流行地区的50只犬的109份血清中IgG1和IgG2的独特型表达，以检测与婴儿利什曼原虫感染及临床状况（无症状或有症状）相关的标志物。来自无利什曼病地区的24只犬用作对照。有症状和无症状的婴儿利什曼原虫感染中IgG1和IgG2反应主要在亚类产生（ELISA值）方面存在差异，较高的IgG2产生尤其出现在有症状的犬中。然而，我们观察到这些IgG亚类的独特型表达差异不大，总体上它们识别相同的抗原组分。婴儿利什曼原虫早期感染的特征是识别低分子量的多肽组分，主要是IgG1识别的14、16和18 kDa组分以及IgG2识别的14和16 kDa组分，而症状则与IgG两个亚类对24 kDa组分和属于AG24家族的其他抗原的识别有关。
