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由无缺陷腺病毒2-猴病毒40杂交体诱导产生的猴病毒40特异性核糖体结合蛋白。

Simian virus 40-specific ribosome-binding proteins induced by a nondefective adenovirus 2-simian virus 40 hybrid.

作者信息

Jay G, Jay F T, Friedman R M, Levine A S

出版信息

J Virol. 1977 Sep;23(3):692-9. doi: 10.1128/JVI.23.3.692-699.1977.

DOI:10.1128/JVI.23.3.692-699.1977
PMID:197266
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC515881/
Abstract

We have studied the intracellular distribution of the two simian virus 40-specific proteins, with apparent molecular weights of 56,000 and 42,000, detectable in human KB cells infected by a nondefective adenovirus 2-simian virus 40 hybrid, Ad2+ND2. After a 20-min pulse of [35S]methionine, about two-thirds of the newly synthesized 56K protein and one-third of the 42K protein were found localized on the plasma membrane. The remainder of each protein was found in the cytoplasm, whereas the nuclear fraction was virtually free of either component. A significant portion of both proteins present in the cytoplasmic fraction was complexed to the 40S ribosomal subunits and was not removed by treatment with 0.5 M KCl. Moreover, the portion that was found free in the cytoplasm could bind preferentially and quantitatively to purified 40S ribosomes in vitro, leading us to propose that these simian virus 40 proteins may act as translational control elements in cells.

摘要

我们研究了两种猿猴病毒40特异性蛋白的细胞内分布情况，这两种蛋白的表观分子量分别为56,000和42,000，在被无缺陷腺病毒2-猿猴病毒40杂交体Ad2+ND2感染的人KB细胞中可检测到。在用[35S]甲硫氨酸脉冲标记20分钟后，发现新合成的56K蛋白约三分之二以及42K蛋白约三分之一定位于质膜上。每种蛋白的其余部分存在于细胞质中，而核部分几乎不含这两种成分。细胞质部分中存在的这两种蛋白的很大一部分与40S核糖体亚基结合，并且用0.5M KCl处理不能将其去除。此外，在细胞质中游离的部分在体外可优先且定量地与纯化的40S核糖体结合，这使我们提出这些猿猴病毒40蛋白可能在细胞中作为翻译控制元件发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e6/515881/160e4d219c3b/jvirol00213-0261-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e6/515881/b995f96447d6/jvirol00213-0258-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e6/515881/cc99569f2d8a/jvirol00213-0259-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e6/515881/7528a9d97967/jvirol00213-0260-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e6/515881/160e4d219c3b/jvirol00213-0261-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e6/515881/b995f96447d6/jvirol00213-0258-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e6/515881/cc99569f2d8a/jvirol00213-0259-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e6/515881/7528a9d97967/jvirol00213-0260-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e6/515881/160e4d219c3b/jvirol00213-0261-a.jpg

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引用本文的文献

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Subcellular localization of the simian virus 40 agnoprotein.猿猴病毒40小ag蛋白的亚细胞定位

本文引用的文献

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NEW SURFACE ANTIGEN IN CELLS TRANSFORMED BY SIMIAN PAPOVAVIRUS SV40.由猴乳头瘤空泡病毒SV40转化的细胞中的新表面抗原
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SYNTHESIS OF SV40 TUMOR ANTIGEN DURING REPLICATION OF SIMIAN PAPOVAVIRUS (SV40).猴乳头瘤空泡病毒（SV40）复制过程中SV40肿瘤抗原的合成
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IMMUNITY IN HAMSTERS TO CELLS TRANSFORMED IN VITRO AND IN VIVO BY SV40. TESTS FOR ANTIGENIC RELATIONSHIP AMONG THE PAPOVAVIRUSES.仓鼠对经SV40体外和体内转化的细胞的免疫性。乳头多瘤空泡病毒之间抗原关系的检测。
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Amount of viral DNA in the genome of cells transformed by adenovirus type 2.2型腺病毒转化细胞基因组中的病毒DNA量。
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