Lavergne J A, del Llano A M
School of Medicine, University of Puerto Rico, Río Piedras.
Pathobiology. 1990;58(2):107-17. doi: 10.1159/000163572.
The in vitro measurement of lymphoid cell activation is a valid correlate of immunological function in human subjects. This process can be evaluated by the assessment of the proliferative response or the cytokine synthesis and secretion by lymphoid cells upon their proper stimulation. The technology of flow cytometry provides unique conditions for the development of biological assays to achieve those purposes. In this work we describe the utilization of flow cytometric DNA analysis in the evaluation of: (1) the mitogenic response of lymphoid cells; (2) the cellular activity in mixed lymphocyte cultures; (3) the presence of interleukin-2 in culture supernatants, and (4) the cell cycle progression, simultaneous to the expression of a nuclear activation antigen among proliferating lymphocytes.
体外测量淋巴细胞活化是人体免疫功能的有效关联指标。该过程可通过评估淋巴细胞在适当刺激后增殖反应或细胞因子合成与分泌来进行评价。流式细胞术技术为开发实现这些目的的生物学检测方法提供了独特条件。在本研究中，我们描述了流式细胞术DNA分析在以下评估中的应用：（1）淋巴细胞的促有丝分裂反应；（2）混合淋巴细胞培养中的细胞活性；（3）培养上清液中白细胞介素-2的存在情况；以及（4）增殖淋巴细胞中细胞周期进程与核活化抗原表达的同步情况。
