固定化里氏木霉纤维素酶对纤维素的水解作用。

Cellulose hydrolysis by immobilized Trichoderma reesei cellulase.

机构信息

Department of Chemical Engineering, Kingsbury Hall, University of New Hampshire, Durham, NH 03824, USA.

出版信息

Biotechnol Lett. 2010 Jan;32(1):103-6. doi: 10.1007/s10529-009-0119-x.

PMID:19731044

Abstract

Cellulose hydrolysis by immobilized Trichoderma reesei cellulase in the presence of a low viscosity ionic liquid, 1-ethyl-3-methylimidazolium diethyl phosphate (EMIM-DEP), was investigated. Preparation of the carrier-free immobilized cellulase was optimized with respect to concentration of the cross-linker and the type of precipitant. The addition of 2% (v/v) EMIM-DEP during hydrolysis gave an initial reaction rate 2.7 times higher than the hydrolysis rate with no ionic liquid. The initial yield after 2 h was 0.7 g glucose/g cellulose, and the carrier-free immobilized cellulase (CFIC) was effectively re-used five times.

摘要

在低黏度离子液体 1-乙基-3-甲基咪唑二乙基磷酸酯（EMIM-DEP）存在的条件下，研究了固定化里氏木霉纤维素酶对纤维素的水解作用。考察了交联剂浓度和沉淀剂种类对无载体固定化纤维素酶制备的优化作用。水解过程中添加 2%（v/v）的 EMIM-DEP，初始反应速率比无离子液体时提高了 2.7 倍。2 h 后的初始产率为 0.7 g 葡萄糖/g 纤维素，无载体固定化纤维素酶（CFIC）可有效重复使用 5 次。