改良法国压力细胞以提高微生物细胞破碎效率。

Modification of a French pressure cell to improve microbial cell disruption.

机构信息

Department of Microbiology and Immunology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, Canada.

出版信息

Photosynth Res. 2009 Oct;102(1):95-7. doi: 10.1007/s11120-009-9493-4.

DOI:10.1007/s11120-009-9493-4

PMID:19731071

Abstract

A procedure for modification of the valve stem of a 40 K French pressure cell is described. The modification should be done by a machinist and requires a metalworking lathe. After modification of the valve stem, a torlon 4203 plastic ball is used between the valve stem and valve seat to control the pressure within the cell. The torlon plastic ball is a key component needed to obtain the high pressures required for efficient disruption of microbial cells.

摘要

介绍了一种修改 40K 法国压力池阀杆的方法。该修改应由机械师完成，需要使用金属车床。修改阀杆后，在阀杆和阀座之间使用 torlon 4203 塑料球来控制池内压力。torlon 塑料球是获得高效破坏微生物细胞所需高压的关键部件。