Huff J W
Bethesda Eye Institute, St. Louis University School of Medicine, MO 63110.
Invest Ophthalmol Vis Sci. 1990 Jul;31(7):1288-93.
The relationship of contact lens-induced edema to epithelial and endothelial function was determined in isolated superfused rabbit corneas. Placement of a polymethyl methacrylate (PMMA) contact lens on the cornea caused swelling rates of 15-28 microns/hr compared to 0-6 microns/hr in paired control corneas. The edema increased with temperature (P less than 0.01). PMMA-induced swelling was significant in: 1) bicarbonate-free Ringer's solution; 2) chloride-free Ringer's; 3) 0.3 mM furosemide-treated corneas; and 4) deepithelialized corneas. The swelling did not occur in corneas with silicone oil replacing the endothelium to block fluid uptake. The effluent aqueous bathing fluid from edematous corneas did not induce edema in normoxic corneas. These studies demonstrate that contact lens-induced edema depends on metabolism, involves a significant stromal contribution, and requires fluid absorption across the endothelial layer, but is not a direct result of epithelial and endothelial ion transport inhibition.
在离体灌注兔角膜中确定了隐形眼镜诱导的水肿与上皮和内皮功能的关系。将聚甲基丙烯酸甲酯（PMMA）隐形眼镜放置在角膜上会导致肿胀速率为15 - 28微米/小时，而配对的对照角膜肿胀速率为0 - 6微米/小时。水肿随温度升高而增加（P < 0.01）。PMMA诱导的肿胀在以下情况中显著：1）无碳酸氢盐的林格氏液；2）无氯林格氏液；3）0.3 mM速尿处理的角膜；4）去上皮角膜。用硅油替代内皮以阻止液体吸收的角膜未出现肿胀。来自水肿角膜的流出房水冲洗液在正常氧合角膜中未诱导水肿。这些研究表明，隐形眼镜诱导的水肿依赖于代谢，涉及基质的显著作用，并且需要液体通过内皮层吸收，但不是上皮和内皮离子转运抑制的直接结果。
