Department of Micro- and Nanotechnology, Technical University of Denmark, Frederiksborgvej 399, DK-4000 Roskilde, Denmark.
Langmuir. 2010 Jan 19;26(2):938-42. doi: 10.1021/la902409n.
Competitive protein adsorption from human serum onto unmodified polyethylene terephthalate (PET) surfaces and plasma-polymerized PET surfaces, using the monomer diethylene glycol vinyl ether (DEGVE), has been investigated using radioactive labeling. Albumin and immunoglobulin G (IgG) labeled with two different iodine isotopes have been added to human serum solutions of different concentrations, and adsorption has been performed using adsorption times from approximately 5 s to 24 h. DEGVE surfaces showed indications of being nonfouling regarding albumin and IgG adsorption during competitive protein adsorption from diluted human serum solutions with relatively low protein concentrations, but the nonfouling character was weakened when less diluted human serum solutions with higher protein concentrations were used. The observed adsorption trend is independent of adsorption time, indicating that the protein concentration has a stronger influence on observed adsorption characteristics of the material than the adsorption time has.
使用放射性标记研究了人血清中未修饰的聚对苯二甲酸乙二醇酯（PET）表面和等离子体聚合 PET 表面上的竞争蛋白吸附，使用了单体二甘醇乙烯基醚（DEGVE）。用两种不同的碘同位素标记的白蛋白和免疫球蛋白 G（IgG）已添加到不同浓度的人血清溶液中，并使用约 5 秒至 24 小时的吸附时间进行了吸附。在从稀释的人血清溶液中进行竞争蛋白吸附时，DEGVE 表面在白蛋白和 IgG 吸附方面表现出非污染的迹象，这些溶液中蛋白质浓度相对较低，但当使用蛋白质浓度较高的稀释程度较低的人血清溶液时，非污染特性会减弱。观察到的吸附趋势与吸附时间无关，这表明蛋白质浓度对材料的观察到的吸附特性的影响比吸附时间更强。
