Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Denmark.
J Appl Microbiol. 2010 Apr;108(4):1235-43. doi: 10.1111/j.1365-2672.2009.04518.x. Epub 2009 Aug 11.
The aim of the present investigation was to identify and characterize Pasteurella-like isolates obtained from clinically affected psittacine birds.
A total of 37 isolates from psittacine birds tentatively classified with the family Pasteurellaceae were characterized phenotypically. The genetic relationship was investigated by sequencing of partial rpoB and 16S rRNA genes for selected isolates. The results obtained were compared with the data from 16 reference strains. Nine isolates were identified as Gallibacterium spp., 16 as Volucribacter spp. or Volucribacter-like, while 11 isolates were classified as taxon 44 of Bisgaard. A single isolate was identified as Pasteurella multocida.
Characterization of Pasteurellaceae by traditional methods is often inconclusive because of inconsistent reactions and phenotypic diversity. For the same reason, genotyping is essential to allow proper classification as demonstrated in the present study.
Limited information exists on the isolation and significance of Pasteurellaceae associated with clinically affected psittacine birds showing signs of digestive and/or respiratory disorders. The present investigations demonstrated that these organisms are widely distributed among clinically affected birds, but isolation of these taxa cannot be unambiguously correlated with the symptoms observed.
本研究旨在鉴定和描述从临床发病的鹦鹉鸟中获得的巴斯德氏菌样分离株。
对暂分类为巴斯德氏菌科的 37 株来自鹦鹉鸟的分离株进行了表型特征分析。选择部分 rpoB 和 16S rRNA 基因进行测序，以研究遗传关系。将获得的结果与 16 株参考菌株的数据进行比较。9 株分离株被鉴定为加氏菌属，16 株为鲸杆菌属或鲸杆菌样属，而 11 株被分类为 Bisgaard 分类群 44。一株分离株被鉴定为多杀巴斯德氏菌。
由于反应不一致和表型多样性，传统方法对巴斯德氏菌科的特征描述往往不明确。出于同样的原因，基因分型对于允许正确分类至关重要，如本研究所示。
有关与表现出消化和/或呼吸障碍症状的临床发病鹦鹉鸟相关的巴斯德氏菌科的分离和意义的信息有限。本研究表明，这些生物体在临床发病的鸟类中广泛分布，但这些分类群的分离不能明确与观察到的症状相关。
