利用基因组步移法研究菠菜 Lhcb1 多基因家族。

Application of a genome walking method for the study of the spinach Lhcb1 multigene family.

机构信息

Department of Biochemistry and Molecular Biology, University of Bari, Via Amendola 165/A, 70126 Bari, Italy.

出版信息

J Plant Physiol. 2010 Jan 15;167(2):138-43. doi: 10.1016/j.jplph.2009.06.020.

DOI:10.1016/j.jplph.2009.06.020

PMID:19732990

Abstract

We describe the application of a novel genome walking (GW) strategy for the one-shot identification of members of multigene families. The method was used to study the spinach Lhcb1 family (encoding the light harvesting complex protein Lhcb1), for which three cDNAs were known. Two additional genes and regulatory regions of the five members of the family were identified. For one of the newly detected genes, sequencing of full-length cDNA and analysis of regulatory regions by gel-shift are also reported. To our best knowledge, this is the first report on the use of a GW approach for the study of a multigene family.

摘要

我们描述了一种新颖的基因组步移（GW）策略在一次鉴定多基因家族成员中的应用。该方法用于研究菠菜 Lhcb1 家族（编码光捕获复合物蛋白 Lhcb1），已知该家族有三个 cDNA。鉴定了该家族的另外两个基因和五个成员的调控区。对于新检测到的基因之一，还报告了全长 cDNA 的测序和凝胶迁移分析的调控区分析。据我们所知，这是首次报道使用 GW 方法研究多基因家族。