Ju Ying, Hao Huai-Jie, Xiong Guo-Hua, Geng Hong-Ran, Zheng Yu-Ling, Wang Jing, Cao Yuanyin, Yang Yin-Hui, Cai Xue-Hui, Jiang Yong-Qiang
State Key Laboratory of Pathogen and Biosecurity, The Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Fengtai District, Beijing 100071, People's Republic of China.
Vet Immunol Immunopathol. 2010 Feb 15;133(2-4):207-11. doi: 10.1016/j.vetimm.2009.08.010. Epub 2009 Aug 19.
To develop colloidal gold immunochromatographic strips for the direct detection of the Streptococcus suis serotype 2 antigen, colloidal gold was prepared by reduction of a gold salt with sodium citrate and coupled with polyclonal antibody against S. suis serotype 2. The optimal concentrations of the capture antibody and the coating antibody were determined to be 22mug/mL and 2.0mg/mL, respectively, and that of the blocking buffer was determined to be 1.5% bovine serum albumin. Different serotypes of S. suis and other related bacteria were used to evaluate the sensitivity, specificity, and stability of the immunochromatographic strips. The detection sensitivity was found to be as high as 10(6)CFU/mL. There was no cross-reaction of the antibodies with other serotypes of S. suis (except with SS1/2, which shares some common sugar residues or antigenic determinants with serotype 2) and other related bacteria. In conclusion, we developed colloidal gold immunochromatographic strips that had high sensitivity and specificity. This method proved to be feasible, convenient, rapid, and effective for detecting S. suis serotype 2.
为开发用于直接检测猪链球菌2型抗原的胶体金免疫层析试纸条，通过用柠檬酸钠还原金盐制备胶体金，并将其与抗猪链球菌2型多克隆抗体偶联。确定捕获抗体和包被抗体的最佳浓度分别为22μg/mL和2.0mg/mL，封闭缓冲液的最佳浓度为1.5%牛血清白蛋白。使用不同血清型的猪链球菌和其他相关细菌评估免疫层析试纸条的敏感性、特异性和稳定性。发现检测灵敏度高达10(6)CFU/mL。抗体与其他血清型的猪链球菌（除与2型共享一些共同糖残基或抗原决定簇的SS1/2外）和其他相关细菌无交叉反应。总之，我们开发了具有高灵敏度和特异性的胶体金免疫层析试纸条。该方法被证明对于检测猪链球菌2型是可行、方便、快速且有效的。
