阿霉素耐药性Lewis肺癌变体的分离与鉴定

Isolation and characterization of doxorubicin-resistant Lewis lung carcinoma variants.

作者信息

Bruno N A, Carver L A, Slate D L

机构信息

Department of Tumor Biology, Syntex Research, Palo Alto, CA 94304.

出版信息

Cancer Commun. 1990;2(4):151-8. doi: 10.3727/095535490820874542.

DOI:10.3727/095535490820874542

PMID:1973357

Abstract

A series of drug-resistant variants of the murine Lewis lung carcinoma (3LL-CK) cell line has been isolated using stepwise selection in increasing concentrations of doxorubicin. These variants exhibited classical multidrug resistance as evidenced by decreased doxorubicin accumulation, cross-resistance to vinblastine, reversibility of resistance by verapamil, and overexpression of P-glycoprotein. When the doxorubicin-resistant 3LL-CK cell populations were injected into the tail veins of B6D2F1 mice, their metastatic abilities were consistently reduced compared with that of the parental line.

摘要

通过在逐渐增加浓度的阿霉素中进行逐步筛选，已分离出一系列小鼠Lewis肺癌（3LL-CK）细胞系的耐药变体。这些变体表现出典型的多药耐药性，表现为阿霉素积累减少、对长春碱的交叉耐药性、维拉帕米可逆转耐药性以及P-糖蛋白的过表达。当将阿霉素耐药的3LL-CK细胞群体注入B6D2F1小鼠的尾静脉时，与亲代细胞系相比，它们的转移能力持续降低。