通过实时定量 PCR 对前列腺癌进行 miRNA 分析：福尔马林固定石蜡包埋组织与新鲜冷冻组织的比较。

miRNA analysis of prostate cancer by quantitative real time PCR: comparison between formalin-fixed paraffin embedded and fresh-frozen tissue.

机构信息

Laboratory of Medical Investigation-LIM55, Urology Department, University of Sao Paulo Medical School, Sao Paulo, Brazil.

出版信息

Urol Oncol. 2011 Sep-Oct;29(5):533-7. doi: 10.1016/j.urolonc.2009.05.008. Epub 2009 Sep 6.

DOI:10.1016/j.urolonc.2009.05.008

PMID:19734068

Abstract

OBJECTIVE

Micro RNA (miRNA) is a class of small noncoding RNA that plays a major role in the regulation of gene expression, which has been related to cancer behavior. The possibility of analyzing miRNA from the archives of pathology laboratories is exciting, as it allows for large retrospective studies. Formalin is the most common fixative used in the surgical pathology routine, and its promotion of nucleic acid degradation is well known. Our aim is to compare miRNA profiles from formalin-fixed paraffin embedded (FFPE) tissues with fresh-frozen prostate cancer tissues.

METHODS

The expression of 14 miRNAs was determined by quantitative real time polymerase chain reaction (qRT-PCR) in 5 paired fresh-frozen and FFPE tissues, which were representative of prostate carcinoma.

RESULTS

There was a very good correlation of the miRNA expression of miR-let7c and miR-32 between the fresh-frozen and FFPE tissues, with Pearson's correlation coefficients of 0.927 (P = 0.023) and 0.960 (P = 0.010), respectively. For the remaining miRNAs, the correlation was good with Spearman correlation coefficient of 0.638 (P < 0.001).

CONCLUSION

Analysis of miRNAs from routinely processed and stored FFPE prostate tissue is feasible for some miRNAs using qRT-PCR. Further studies should be conducted to confirm the reliability of using stock tissues for miRNA expression determination.

摘要

目的

微小 RNA（miRNA）是一类在基因表达调控中起主要作用的小非编码 RNA，与癌症行为有关。分析病理学实验室档案中的 miRNA 的可能性令人兴奋，因为它可以进行大规模的回顾性研究。福尔马林是外科病理学常规中最常用的固定剂，其促进核酸降解是众所周知的。我们的目的是比较福尔马林固定石蜡包埋（FFPE）组织和新鲜冷冻前列腺癌组织中的 miRNA 谱。

方法

通过定量实时聚合酶链反应（qRT-PCR）在 5 对代表前列腺癌的新鲜冷冻和 FFPE 组织中测定了 14 种 miRNA 的表达。

结果

miR-let7c 和 miR-32 的 miRNA 表达在新鲜冷冻和 FFPE 组织之间具有很好的相关性，Pearson 相关系数分别为 0.927（P = 0.023）和 0.960（P = 0.010）。对于其余 miRNA，Spearman 相关系数为 0.638（P < 0.001），相关性也很好。