Strzezek Rafał, Fraser Leyland
Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego Street 5, 10-718 Olsztyn-Kortowo, Poland.
Reprod Biol. 2009 Jul;9(2):113-26.
This study aimed to analyze the effects of different osmolalities on the characteristics of spermatozoa originating from whole ejaculates (WE; including the prostatic fluid) and the sperm-rich fractions (SRF). Ejaculates, collected from four mixed-breed dogs, were exposed for 10 min at room temperature to Tris-fructose-citrate (TFC) solution with osmolality ranging from 150 to 1100 mOsm. After treatment spermatozoa were evaluated by microscopic analysis of motility and fluorescent assessments of plasma membrane integrity (carboxyfluorescein diacetate and propidium iodide, CFDA/PI) and mitochondrial function (rhodamine 123, R123). Irrespective of the sperm source, there was a complete loss of motility when spermatozoa were exposed to TFC solution with 1100 mOsm. There were no marked differences in the sperm characteristics between media with 300 and 350 mOsm, regardless of the ejaculate collection procedure. However, a marked reduction in motility of spermatozoa retrieved either from the WE or SRF was observed after exposure to different anisosmotic conditions (150, 550 and 800 mOsm). In all dogs, spermatozoa from the WE exhibited greater osmotolerance in terms of plasma membrane integrity and mitochondrial function when exposed to anisosmotic conditions (150, 550, 800 and 1100 mOsm). There were inter-dog variations in response to various osmotic conditions. The findings of this study indicated that spermatozoa from the WE tolerated exposure to a wider range of osmolality than those from the SRF. It seemed that the presence of prostatic fluid of dog semen rendered the sperm membrane structures less susceptible to osmotic stress.
本研究旨在分析不同渗透压对来自全精液（WE；包括前列腺液）和富含精子部分（SRF）的精子特征的影响。从四只杂种犬采集的精液在室温下于渗透压范围为150至1100 mOsm的Tris-果糖-柠檬酸盐（TFC）溶液中暴露10分钟。处理后，通过显微镜分析运动性以及对质膜完整性（羧基荧光素二乙酸酯和碘化丙啶，CFDA/PI）和线粒体功能（罗丹明123，R123）进行荧光评估来评价精子。无论精子来源如何，当精子暴露于渗透压为1100 mOsm的TFC溶液时，运动性完全丧失。无论射精采集程序如何，渗透压为300和350 mOsm的培养基之间的精子特征没有明显差异。然而，在暴露于不同的非等渗条件（150、550和800 mOsm）后，观察到从WE或SRF中获取的精子运动性显著降低。在所有犬中，当暴露于非等渗条件（150、550、800和1100 mOsm）时，来自WE的精子在质膜完整性和线粒体功能方面表现出更高的渗透压耐受性。不同犬对各种渗透压条件的反应存在差异。本研究结果表明，与来自SRF的精子相比，来自WE的精子能够耐受更广泛的渗透压范围。犬精液中前列腺液的存在似乎使精子膜结构对渗透压应激的敏感性降低。
