Sjögren Florence, Anderson Chris
Department of Clinicial and Experimental Medicine, Division of Dermatology, University Hospital, Linköping, Sweden.
Acta Derm Venereol. 2009;89(5):459-65. doi: 10.2340/00015555-0683.
Microdialysis allows the study of the local production and temporal resolution of cytokines in living skin. Samples were taken from the normal skin of 10 healthy subjects for 24-28 h after insertion of a concentric microdialysis catheter, and analysed with a Luminex bead-based assay. Interleukin-1 beta (IL1b), IL6 and IL8 were seen in all subjects at all time-points after the first hour. Levels peaked at 5-8 h, equilibrating to lower levels at 24 h. Immunohistological double staining for human leukocyte antigen (HLA)-DR and intracellular cytokines on biopsies taken after catheter removal showed many stained cells in the dermis, in contrast to the few cells stained in the epidermis. This study demonstrates the reactive capability of the dermis when provoked separately from the epidermis. The production of IL1b, IL6 and IL8 occurs invariably in what can be termed an innate, dermal response to "danger"; in this case in the form of sterile needle trauma.
微透析技术可用于研究活皮肤中细胞因子的局部产生及时间分辨率。在插入同心微透析导管后，从10名健康受试者的正常皮肤中采集样本24 - 28小时，并采用基于Luminex微球的检测方法进行分析。在第一个小时后的所有时间点，所有受试者均检测到白细胞介素-1β（IL1b）、IL6和IL8。水平在5 - 8小时达到峰值，在24小时时降至较低水平。在拔除导管后采集的活检组织上进行人白细胞抗原（HLA）-DR和细胞内细胞因子的免疫组织化学双重染色显示，真皮中有许多染色细胞，而表皮中染色细胞较少。本研究证明了真皮在与表皮分离激发时的反应能力。IL1b、IL6和IL8的产生总是以对“危险”的固有真皮反应形式发生；在这种情况下，是以无菌针创伤的形式。
