HLA Typing Laboratory, Blood Center of Zhejiang Province, Hangzhou, Zhejiang Province 310006, China.
Int J Immunogenet. 2009 Dec;36(6):351-60. doi: 10.1111/j.1744-313X.2009.00874.x. Epub 2009 Sep 7.
To analyse the complete genomic sequences and investigate the intron polymorphism of the human leucocyte antigen (HLA)-A locus, the full-length nucleotide sequences of each major allelic group of HLA-A in the Chinese Han population were determined, including HLA-A01, A02, A03, A11, A23, A24, A26, A29, A30, A31, A32, A33, A34, A68, A69. More than 3.0-kb DNA fragment of HLA-A locus was amplified from 5'-untranslated region to 3'-noncoding region for sequencing. Full-length sequences of the HLA-A alleles were determined using an ABI BigDye((R)) Terminator Cycle Sequencing kit and the HLA-A phylogenetic tree was analysed by dnaman software. Full-length nucleotide sequences of 15 HLA-A alleles (GenBank Accession numbers EU445470-EU445484) were obtained. HLA-A110101, A2301, A300101, A310102, A330301, A340101, A680102 and A*6901 alleles were firstly reported for complete genomic sequences. Total 247 polymorphism positions were found in the complete genomic sequences of HLA-A alleles and a insertion of 17 nucleotides within intron 3 was observed in several allelic groups. According to the phylogenetic tree of the full-length nucleotide sequences, HLA-A locus was classified into seven major allelic lineages. In this study, complete genomic sequences of common HLA-A alleles were obtained and the data will help us understand the evolution of HLA-A.
为分析人类白细胞抗原（HLA）-A 座的完整基因组序列并研究内含子多态性，本研究确定了中国汉族人群中 HLA-A 主要等位基因组的全长核苷酸序列，包括 HLA-A01、A02、A03、A11、A23、A24、A26、A29、A30、A31、A32、A33、A34、A68、A69。从 5'-非翻译区扩增 HLA-A 座超过 3.0-kb 的 DNA 片段用于测序。使用 ABI BigDye((R))终止子循环测序试剂盒确定 HLA-A 等位基因的全长序列，并使用 dnaman 软件分析 HLA-A 系统发育树。获得了 15 个 HLA-A 等位基因（GenBank 登录号 EU445470-EU445484）的全长核苷酸序列。首次报道了 HLA-A110101、A2301、A300101、A310102、A330301、A340101、A680102 和 A*6901 等位基因的完整基因组序列。在 HLA-A 等位基因的完整基因组序列中发现了 247 个多态性位置，并在几个等位基因组中观察到内含子 3 内的 17 个核苷酸插入。根据全长核苷酸序列的系统发育树，HLA-A 座分为七个主要等位基因谱系。本研究获得了常见 HLA-A 等位基因的完整基因组序列，这些数据将有助于我们了解 HLA-A 的进化。
