Hibbard Bruce E, El Khishen Ahmed A, Vaughn Ty T
USDA-ARS, Plant Genetics Research Unit, 205 Curtis Hall, University of Missouri, Columbia, MO 65211, USA.
J Econ Entomol. 2009 Aug;102(4):1607-13. doi: 10.1603/029.102.0427.
The effects of maize, Zea mays L., phenology on establishment, damage, and adult emergence of the western corn rootworm, Diabrotica virgifera virgifera LeConte, on MON863 transgenic maize expressing the Cry3Bb1 protein and its isoline was evaluated in field trials in 2002 and 2003. As expected, plant damage, western corn rootworm larval recovery, and adult emergence were significantly lower on MON863 than isoline maize. The average weight of larvae and adults recovered from MON863 and isoline maize was generally not significantly different. If western corn rootworm larvae were able to establish on transgenic rootworm-resistant plants, larval growth was relatively normal. Plant damage, the number of western corn rootworm larvae recovered, and adult emergence from MON863 did not significantly differ between egg hatch times from widely varying phenologies in either year of the study. Although the extractable level of Cry3Bb1 decreased significantly from vegetative (V)4 to V9 maize in previous studies, in the current study, the amount of Cry3Bb1 did not vary from V3 to R3 in a way that affected neonate survival by western corn rootworm larvae in the field.
2002年和2003年在田间试验中评估了玉米（Zea mays L.）物候对西部玉米根萤叶甲（Diabrotica virgifera virgifera LeConte）在表达Cry3Bb1蛋白的MON863转基因玉米及其近等基因系上的定殖、危害和成虫羽化的影响。正如预期的那样，MON863上的植株危害、西部玉米根萤叶甲幼虫回收率和成虫羽化率显著低于近等基因系玉米。从MON863和近等基因系玉米中回收的幼虫和成虫的平均重量通常没有显著差异。如果西部玉米根萤叶甲幼虫能够在转基因抗根萤叶甲植物上定殖，幼虫生长相对正常。在研究的任何一年中，不同物候期的卵孵化时间对MON863上的植株危害、回收的西部玉米根萤叶甲幼虫数量和成虫羽化率均无显著差异。尽管在先前的研究中，从营养生长（V）4期到V9期玉米，Cry3Bb1的可提取水平显著下降，但在本研究中，Cry3Bb1的量从V3期到R3期并未以影响田间西部玉米根萤叶甲幼虫新生幼虫存活的方式发生变化。
